摘要As a pathological feature of diabetic kidney disease(DKD),dysregulated glomerular filtration barrier function could lead to the increased levels of proteinuria.The integrity of tight junctions(TJs)of glomerular endothelial cells(GECs)is a guarantee of physiological function of glomerular filtration barrier.Mammalian sterile 20-like kinase(MST1)is a key regulatory protein in the blood-brain barrier(BBB),and it regulates the expression of TJs-related proteins in cerebral vascular endothelial cells.Our previous study showed that MST1 was involved in renal tubulointerstitial fibrosis of DKD.In the present study we investigated the role of MST1 in barrier function of GECs of DKD,and explored its regulatory mechanisms.In kidney tissue section of DKD patients and db/db mice,and high glucose(HG)-cultured mouse glomerular endothelial cells(mGECs),we showed that MST1 was inactivated in the GECs of DKD accompanied by disrupted glomerular endothelial barrier.In db/db mice and HG-cultured mGECs,knockdown of MST1 increased proteinuria levels,and disrupted glomerular endothelial barrier through decreasing TJs-related proteins,whereas MST1 overexpression restored glomerular endothelial barrier through regaining TJs-related proteins.In db/db mice and HG-cultured mGECs,we demonstrated that MST1 inhibition induced TJs's disruption of GECs via activating YAP1/TEAD signaling.Verteporfin(an inhibitor of YAP1-TEAD interaction)and PY-60(a YAP1 agonist)were used to verify the role of YAP1/TEAD signaling in the regulation effect of MST1 on barrier function of mGECs.In conclusion,MST1 activation recovers glomerular endothelial barrier of DKD by regaining TJs-related proteins via inhibiting YAP1/TEAD signaling.This study highlights the multiple regulation of MST1 activation on kidney injury.