摘要目的 观察加味补肝汤对糖尿病大鼠坐骨神经中VEGF mRNA表达的影响,探讨其对实验性糖尿病大鼠周围神经病变的防治作用.方法 以链脲佐菌素诱发糖尿病模型.分别于治疗后4周、8周两个时间点处死大鼠,用逆转录-聚合酶链反应(RT-PCR)技术测定坐骨神经中VEGF mRNA的表达.结果 治疗后4周末、8周末模型组大鼠及加味补肝汤组VEGF mRNA表达均明显高于正常对照组(P＜0.05),其中4周末加味补肝汤组表达较同期模型组升高显著(P＜0.05),而8周末较同期模型组降低显著(P＜0.05).结论 加味补肝汤对实验性糖尿病大鼠周围神经病变有一定的防治作用.

abstractsObjective To observe the effect of jiaweibugan decoction on VEGF expression of sciatic nerve in experimental diabetic rat and explore the preventive and therapeutic effects of iiaweibugan decoction on peripheral neumpathy in~xperimental diabetic rats.Methotis The diabetic rat model was established by streptozotocin(STZ).The rats were killed on the 4th or 8th week from the beginning of treatment respectively,and VEGF mRNA of sciatic nerve was detected by reverse-transcriptase polymerase chain reaction(RT-PCR).Resuits The results of RT-PCR showed that VEGF expression ofthe model group and treated group on the4th or8th week WaS higherthan that of the normal control group(P＜0.05).Compared with the model group,VEGF expression in the diabetic rats treated byjiaweibugan decoction increased markedly at the end of4th week(P＜0.05),while decreaSed significantly at the end of8th week(P＜0.05).Conclusion Jiaweibugan decoction has a proventive and therapeutic effect on peripheral neumpathy in experimental diabetic rats.