摘要目的 探讨热休克蛋白70-2(HSP70-2)基因+1267A/G多态性在中国汉族人群中的分布及与冠心病的相关性.方法 采用聚合酶链反应-限制性片段长度多态性技术,对汉族185例冠心病患者及149名正常人群HSP70-2基因+1267A/G多态性,基因型及等位基因分布进行研究.结果 研究人群中存在HSP70-2基因+1267A/G多态性,基因型符合Hardy-Weinberg平衡,冠心病患者G等位基因频率显著高于对照组(61.89%vs51.68%,P＜0.01).Logistic回归分析得出:HSP70-2基因+1267A/G多态性(AG+GG)基因型是冠心病的独立危险因素,(AG+GG)基因型比AA基因型的OR=2.031,95%CI=1.024～4.026.结论 HSP70-2基因+1267A/G多态性与冠心病有关,G等位基因可能是汉族人群冠心病的遗传危险因素.

abstractsObjective To investigate the correlation between heat-shock protein 70-2 (HSP70-2) gene+1267A/G polymorphism and coronary heart disease (CHD) in Han Chinese population. Methods Using the method of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) , the polymorphism and genotype and allele distribution of HSP70-2 gene + 1267A/G in 185 CHD patients and 149 controls were analyzed. Results The HSP70-2 gene + 1267A/G polymorphism was found in this study population.The distribution of HSP70-2 genotypes was in Hardy-Weinberg equilibrium. The frequency of G allele in CHD group was significantly higher than that in control (61.89% vs51.68%, P ＜ 0. 01). After multiple logistic regression analysis, HSP70-2 gene (GG + AG) genotype was an independent risk factor of coronary heart disease. Conclusion HSP70-2 gene + 1267A/G polymorphism was associated with CHD risk of Han Chinese population, the G allele might serve as a genetic risk factor of coronary heart disease.