摘要目的 检测糖尿病肾病患者血清蛋白氧化损伤指标的变化,探讨蛋白氧化损伤在糖尿病肾病发病中的可能机制.方法 应用改良后的Witko-Sarsat法和2,4-二硝基苯肼法检测血清晚期氧化蛋白产物(AOPP)水平、蛋白质羰基(PCO)含量,应用ELISA及酶谱法分别检测血清基质金属蛋白酶-2(MMP-2)的表达水平和活性.结果 DN1组、DN2组AOPP水平明显高于DM组(78.23±19.30 vs 61.25±12.13、101.59±30.22 vs 61.25±12.13,F＝41.988,P＜0.01),DN1组、DN2组PCO含量亦明显高于DM组(0.84±0.03 vs 0.66±0.02、1.05±0.05 vs 0.66±0.02,F＝205.763,P＜0.01),AOPP、PCO与血清MMP-2的表达水平(r＝0.460,0.480,P＜0.05)和活性(r＝0.385,0.560,P＜0.05)均呈正相关,多元逐步回归分析表明AOPP、PCO为血清MMP-2表达水平(P＜0.05,P＜0.01)和活性(P＜0.01,P＜0.05)的主要影响因素.结论 推测蛋白氧化损伤通过改变MMP-2的表达和活性,影响肾脏基质代谢.

abstractsObjective Serum indicators of oxidative protein damage (OPD) were analyzed to explore the effect on renal MMP/TIMP system of OPD in diabetic nephropathy. Methods AOPP levels and PCO levels were measured by modified Witko-Sarsat's method and 2, 4-dinitrophenylhydrazine spectrophotometric method, expression of serum MMP-2 were determined by ELISA, and MMP-2 activity were detected by zymography . Results AOPP levels of group DN1, group DN2 were higher than those of group DM(78.23±19.30 vs 61.25±12.13,101.59±30.22 vs 61.25±12.13,F＝41.988,P＜0.01). PCO levels of group DN1 and group DN2 were higher than those of group DM (0.84±0.03 vs 0.66±0.02,1.05±0.05 vs 0.66±0.02,F＝205.763,P＜0.01). Pearson correlation analysis indicated AOPP and PCO were positively correlated with the expression (r＝0.460,0.480,P＜0.05) and activity (r＝0.385,0.560,P＜0.05) of serum MMP-2. Multiple stepwise regression analysis showed that AOPP and PCO were major influential factors of serum MMP-2 expression (P＜0.05,P＜0.01) and activity(P＜0.01,P＜0.05). Conclusions OPD might be involved in the imbalance of renal matrix metabolism, which was correlated with the development of diabetic nephropathy.