摘要目的 观察猪自体骨髓单个核细胞移植后心肌梗死区干细胞因子、基质细胞衍生因子-1、E-选择素、细胞间粘附分子-1、血管细胞粘附因子-1等细胞因子mRNA的表达.方法 前降支球囊封堵法建立10头猪急性心肌梗死动物模型,按随机数字表法分为对照组(5头)和移植组(5头).造模1周后,移植组冠脉内移植自体骨髓单个核细胞,对照组注射1640培养基作为对照.6个月后取梗死区心脏组织观察局部细胞因子的mRNA表达.结果 移植组梗死区能检测到基质细胞衍生因子-1、干细胞因子、E-选择素、细胞间粘附分子-1、血管细胞粘附分子-1等细胞因子的mRNA.其表达强度明显高于对照组.结论 干细胞移植能促进局部的基质细胞衍生因子-1、干细胞因子、E-选择素、细胞间粘附分子-1和血管细胞粘附分子-1 mRNA的表达.

abstractsObjective To investigate the effects of bone marrow stem cells autologous transplantation after acute myocardial infarction on local mRNA expression of SDF-1,Kit ligand,E-selectin,ICAM-1and VCAM-1.Methods Acute myocardial infarction models were established in 10 swine,which were randomized divided into placebo group (5 swine) and transplantation group (5 swine).After six months,hearts of placebo group and early transplantation group were harvested and mRNA expression of SDF-1,Kit ligand,E-selectin,ICAM-1 and VCAM-1 in infarcted myocardial region was detected.Results The mRNAs of SDF-1,Kit ligand,E-selection,ICAM-1 and VCAM-1 were expressed in the infarcted myocardial region of transplantation group six months after myocardial infarction,but it was not found in the placebo group.Conclusions Bone marrow autologous transplantation after acute myocardial infarction can promote local mRNA expression of SDF-1,Kit ligand,E-selectin,ICAM-1 and VCAM-1,which may be due to the paracrine effects of stem cells.