摘要目的 探讨膀胱尿路上皮癌中MHC Ⅰ类链相关蛋白A(MHC classⅠ chain-related A,MICA)的表达,以及与核因子-κB(nuclear factor-κB,NF-κB)和p53的相互关系,为研究膀胱癌组织中MICA蛋白的表达机制提供组织学依据.方法 用免疫组化方法检测75例膀胱尿路上皮癌及15例正常膀胱黏膜中MICA、NF-κB和p53蛋白表达,对MICA、NF-κB和p53在正常膀胱黏膜、浸润和非浸润膀胱癌中的表达进行统计学分析.结果 (1)MICA、NF-κB和p53蛋白在膀胱癌的表达率分别为48.0％、85.3％和49.3％,均显著高于正常膀胱黏膜(P＜0.05).MICA蛋白在浸润性膀胱癌的表达低于非浸润性膀胱癌(P＜0.05).(2)膀胱癌组织中MICA与NF-κB蛋白表达呈正相关(r=0.256,P=0.027),而MICA和p53蛋白的表达呈负相关(r=-0.23,P=0.047).结论 膀胱癌中MICA蛋白常表达上调,可作为膀胱癌的肿瘤相关抗原；NF-κB通路可能参与MICA的表达调控；p53通路可能不参与膀胱尿路上皮恶性转化过程中MICA蛋白的表达.

abstractsObjective To investigate the mechanism of the expression of MHC class Ⅰ chain-related A (MICA) in carcinoma of the urinary bladder,the relationship between MICA,nuclear factor-κB ( NF-κB) and p53 expression in bladder cancer was studied.Methods The expression of MICA,NF-κB and p53 in a total of 75 cases of urothelial carcinoma tissues and 15 normal mucous membrane tissues of the bladder was evaluated by immunohistochemistry.Results The expression rates of MICA,NF-κB and p53 protein in urothelial carcinoma were 4.08％,85.3％ and 49.3％,respectively.Up-regulation of their expression was found in urothelial carcinoma compared with normal mucous membrane tissues ( P ＜ 0.05 ).MICA expression was positively correlated with NF-κB expression( r =0.256,P =0.027),but negatively correlated with p53 expression( r =- 0.23,P =0.047 ).Conclusions Up-regulation of MICA expression was detected in bladder cancer.MICA protein may be a new tumor-associated antigen of bladder cancer.MICA expression may be regulated by NF-κB pathway,while p53 pathway may not play a part in MICA up-expression during the urothelial malignant transformation.