摘要目的 研究姜黄素在体外对人胃癌细胞BGC-823生长增殖的影响及其抗肿瘤的相关分子机制.方法 体外培养人胃癌BGC-823,MTT法检测姜黄素在相同浓度下,不同时间对于胃癌细胞增殖的影响,并计算IC50值；RT-PCR法检测其对COX-2、MMP-9 mRNA表达的影响.结果 MTF结果显示:相同干预浓度姜黄素抑制人胃癌细胞增殖,其24、48、72 h的IC50分别为:(62.192±7.802) μmol/L、(46.365±8.991) μmol/L和(45.503±9.126) μmol/L; RT-PCR结果显示:正常对照组及姜黄素干预组的人胃癌细胞BGC-823MMP-9 mRNA灰度值分别为:0.704±0.045,0.147 ±0.052(P＜0.05)；COX-2 mRNA灰度值分别为:0.721 ±0.060,0.190±0.027(P＜0.05).结论 姜黄素抗肿瘤机制可能通过抑制COX-2及MMP-9 mRNA的表达来抑制胃癌细胞增殖.

abstractsObjective To investigate modulating effects of curcumin on proliferation and the molecular mechanism in gastric carcinoma cell line BGC-823.Methods Cells were incubated with curcumin in the same concentration for 24 h,48 h,and 72 h.The cell viability was measured by MTT assay.IC50 was calculated.RT-PCR was used to evaluate the expression of COX-2 and MMP-9.Results The BGC-823 cell viability was apparently inhibited by curcumin with a inhibitory effect in the time-dependent and dosedependent manner.The IC50 of the BGC-823 cell line was (62.192 ±7.802)μmol/L (24 h),(46.365 ±8.991) μmol/L (48 h),and (45.503 ±9.126) μmol/L (72 h).RT-PCR showed the expressions of COX-2 and MMP-9 were down-regulated.The expression of MMP-9 mRNA in the BGC-823 cells was 0.704 ±0.045 for the normal control group and 0.147 ± 0.052 for the curcumin-treated group wit a statistical significance (P ＜ 0.05) ; COX-2 were 0.721 ± 0.060 for the normal control group and 0.190 ± 0.027 for the curcumin-treated group with a statistical significance (P ＜ 0.05).Conclusions The molecular mechanism that curcumin inhibited the proliferation of tumor cells might be that it inhibited preferentially the expressions of COX-2 and MMP-9 in gastric carcinoma cell line.