摘要目的 研究益生菌VSL#3对葡聚糖硫酸钠诱导的大鼠慢性实验性结肠炎预防作用及机制.方法 反复自由饮用5％葡聚糖硫酸钠溶液诱导大鼠慢性结肠炎模型,30只SD大鼠分为3组,正常对照组(n=10),益生菌VSL#3组(n=10),模型组(n=10),实验第69天处死大鼠,评价大肠病理学损伤,应用Real-time PCR方法与ELISA方法分别检测大肠组织以及血清中IFN-γ及IL-4的表达水平.结果 益生菌VSL#3组Hamamoto评分(4.23±1.08)明显低于模型组(6.25±1.36)(P＜0.05)；与模型组相比,益生菌VSL#3组IFN-γ表达水平降低[血清(26.13±5.11) pg/ml vs(40.13±9.15)pg/ml,大肠组织:8.93±1.81 vs 13.27±1.65,P＜0.05]；益生菌VSL#3组IL-4表达水平升高[血清(37.25±6.86) pg/ml vs(24.47±7.93) pg/ml,大肠组织:9.55±2.31 vs7.16±1.19,P ＜0.05].结论 益生菌VSL#3能够通过提高IL-4活性,降低IFN-γ的活性防治DSS诱导的大鼠慢性实验性结肠炎的肠道炎症.

abstractsObjective To explore the effects of probiotic VSL#3 on rat dextran sulfate sodium-induced chronic experimental colitis.Methods Rat colitis was induced by administration of 5％ dextran sulfate sodium solution (freely drink),30 Sprague-Dawley (SD) rats were divided into control group (n =10),VSL#3 group (n =10) and model group (n =10).All rats were killed at 69th day and pathological changes were evaluated.Expression of interferon (IFN)-γ and interleukin-4 (IL-4) in colon tissue and serum were measured by real-time polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA),respectively.Results The colonic inflammation alleviated (Hamamoto score:4.23 ± 1.08 vs 6.25 ± 1.36,P ＜ 0.05) compared with the model group.Compared with model group,the expression of IFN-γwas decreased [serum:[(26.13 ± 5.11) pg/ml] vs (40.13 ± 9.15) pg/ml ; colon tissue:8.93 ± 1.81 vs 13.27 ± 1.65,P ＜ 0.05] and interleukin-4 was increased significantly [serum:(37.25 ± 6.86)pg/ml vs (24.47 ±7.93) pg/ml; colon tissue:9.55 ±0.31 vs 7.16 ± 1.19,P ＜0.05] in probiotic VSL#3 group.Conclusions The probiotic VSL#3 can increase the activity of IL-4 and lower IFN-gamma activity to prevent and treat DSS-induced chronic experimental colitis intestinal inflammation.