摘要目的 观察三七总皂甙对慢性低氧性肺动脉高压大鼠肺动脉病理变化的影响,探讨分析三七总皂甙对防治慢性低氧性肺动脉高压的作用和可能的机制.方法 将40只SD雄性大鼠按随机数字表法分成四组(n=10):正常组(C组),单纯低氧组(H组),低氧+卡托普利组(HC组)和低氧+三七总皂甙组(HP组).H、HC、HP三组建立常压缺氧性大鼠肺动脉高压模型后,经胸直接行肺动脉插管测量各组大鼠肺动脉收缩压及肺动脉平均压力值,最后处理肺动脉观察肺动脉病理改变.结果 (1)经胸肺动脉直接插管法测量出各组大鼠肺动脉内压力:H组肺动脉收缩期压力和平均肺动脉压力与C组差异有统计学意义(P＜0.05);HC、HP组与C组差异无统计学意义(P＞0.05).(2)组织学检查:H组的大鼠肺动脉病理改变相对明显,表现为血管内膜增生、血管平滑肌细胞增殖、中膜肥厚、细胞外基质成分增多、管壁增厚和管腔狭窄等.结论 三七总皂甙具有抑制慢性低氧性肺动脉高压大鼠的肺血管重构作用,具有防治肺动脉高压的效果.

abstractsObjective To observe the effect of panax notoginseng saponins on pulmonary artery pathology in chronic hypoxic pulmonary hypertensionp (HPH) rats models, discuss the role and possible mechanisms of panax notoginseng saponins in prevention of chronic hypoxic pulmonary hypertension.Methods Forty male Sprague-Dawley (SD) rats were randomly divided into four groups (n =10).Group C: control group;Group H: rats were treated with hypoxia only;Group HC: rats were treated with hypoxia and captopril;and Group HP: rats were treated with hypoxia and panax notoginseng saponins.To observe the effect of panax notoginseng saponins prophylactic treatment in chronic hypoxic pulmonary hypertension rats,after the establishment of the model of hypoxic rat animal pulmonary hypertension models, transthoracic direct pulmonary artery intubation was measured in rat pulmonary arterial systolic pressure and mean pulmonary artery pressure, at the end of separation and cut for pulmonary artery to observe the pathological changes of pulmonary artery, results were recorded and statistically analyzed.Results (1)Transthoracic direct pulmonary artery intubation was measured in rat pulmonary arterial systolic pressure and mean pulmonary artery pressure: pulmonary artery systolic pressure (PASP) and mean pulmonary arterial pressure (MPAP) were significantly higher in Group H than in Group C (P ＜ 0.05), Group HP was no significant difference relative to Group C.(2)Pathological section shew that the primary pathological change of group H is vascular intimal hyperplasia and the proliferation of smooth muscle cells, medial hypertrophy, extracellular matrix increased, thickening of the vessel wall and the vascular stenos.Conclusions Panax notoginseng saponins inhibit the formation of chronic hypoxic pulmonary hypertension and pulmonary vascular remodeling in rats,and have the effect of prevention and treatment of pulmonary hypertension.