摘要目的 探讨富氢液是否通过激活血红素氧合酶-1(HO-1)减轻炎性痛大鼠的炎症反应.方法 成年雄性SD大鼠80只,体重180 ～ 220 g,采用随机数字表法,将其分为5组(n=16):对照组(Con组)、炎性痛组(CFA)、炎性痛+富氢液组(CFA+ H2)、炎性痛+HO-1抑制剂Znpp-Ⅸ组(CFA+ Znpp-Ⅸ)、炎性痛+富氢液+HO-1抑制剂Znpp-Ⅸ组(CFA+H2+ Znpp-Ⅸ).分别于模型制作第1天(T1)、第2天(T2)、第3天(T3)、第5天(T4)、第7天(T5)和第14天(T6)检测大鼠疼痛行为学(机械痛敏和热痛敏);于第7天收集大鼠脊髓组织,应用RT-PCR和Westem blot等方法检测HO-1的mRNA、蛋白表达和活性的变化,应用ELISA法检测炎症因子的表达.结果 与对照组比较,CFA组动物机械痛敏MWT和热痛敏TWL降低,脊髓组织中HO-1 mRNA、蛋白表达和活性增加,炎症因子TNF-α、IL-1β、IL-6和IL-10表达增加(P＜0.05);与CFA组比较,CFA+ H2组动物机械痛敏MWT和热痛敏TWL增加,脊髓组织中HO-1 mRNA、蛋白表达和活性进一步增加,炎症因子TNF-α、IL-1β和IL-6表达减少,而IL-10表达进一步增加(P＜0.05);与CFA+ H2组比较,CFA+ H2+Znpp-Ⅸ组动物机械痛敏MWT和热痛敏TWL降低,脊髓组织中HO-1 mRNA、蛋白表达和活性减少,炎症因子TNF-α、IL-1β和IL-6表达增加,而IL-10表达减少(P＜0.05).结论 富氢液可减轻炎性痛大鼠的机械痛敏和热痛敏,降低炎症因子释放,与激活HO-1密切相关.

abstractsObjective To investigate whether hydrogen-rich saline could ameliorate the expressions of cytokines in a rat model of inflammatory pain through activating heme oxygenase-1 (HO-1).Methods Eighty male Sprague-Dawley (SD) rats,weighing 180 ～ 220 g,were randomly divided into five groups (n =16 in each group):Control group (Con),inflammation pain group (CFA),inflammation pain + hydrogen-rich saline group (CFA + H2),inflammation pain + HO-1 inhibitor Znpp-Ⅸ group (CFA + Znpp-Ⅸ),and inflammation pain + hydrogen-rich saline + HO-1inhibitor Znpp-Ⅸ group (CFA + H2 + Znpp-Ⅸ).The mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL) were tested on days 1 (T1),2 (T2),3 (T3),5 (T4),7 (T5),and 14 (T6) after inflammation pain.The expressions of spinal HO-1 mRNA and protein were measured with real-time quantitative polymerase chain reaction (RT-PCR) and Western blot,and spinal inflammatory cytokines were measured with enzyme-linked immunosorbent assay (ELISA) on day 7 after inflammatory pain.Results Compared to Con group,MWT and TWL were significantly reduced;the spinal HO-1 mRNA level,protein expression and activity were increased;and the levels of tumor necrosis factor-α (TNF-α),interleukin (IL)-1β,IL-6 and IL-10 in spinal tissues were also increased in CFA group (P ＜ 0.05).Compared to CFA group,MWT and TWL were significantly increased;the spinal HO-1 mRNA level,protein expression and activity were further increased;and the levels of TNF-α,IL-1β and IL-6 were decreased,while IL-10 was further increased in CFA + H2group (P ＜ 0.05).Compared to CFA + H2 group,MWT and TWL were decreased;the spinal HO-1 mR-NA level,protein expression and activity were decreased;and the levels of TNF-α,IL-1β and IL-6 in spinal tissue were significantly increased,while IL-10 was decreased in CFA + H2 + Znpp-Ⅸ group (P ＜0.05).Conclusions Hydrogen-rich saline can ameliorate the mechanical and thermal allodynia in a rat model of inflammatory pain,and reduce the release of inflammatory cytokines via activating HO-1.