摘要目的 通过癌症和肿瘤基因图谱(TCGA)数据集和基因表达汇编(GEO)数据库探索谷胱甘肽还原酶(GSR)在胃癌中的表达情况,研究GSR与胃癌临床病理特征的联系,分析GSR与其他生物通路的关系,推测GSR对评价胃癌患者预后的意义,探讨GSR在胃癌发生发展过程中的作用.方法 检索TCGA中的胃癌数据集并下载,结合临床信息对芯片数据进行分析.对肿瘤样本进行基因富集分析从而发现与GSR相关的生物学通路.结果 GSR在不同美国肿瘤研究联合委员会(AJCC)T分期中的表达水平差异有统计学意义(P＜0.01).在不同年龄、性别、肿瘤最短径、AJCC M分期、有无Barrett食管、有无胃癌家族史、有无幽门螺杆菌(HP)感染中的表达量则差异无统计学意义(P＞0.05).GSR高表达患者的无病生存期及总生存期均比GSR低表达患者更长(P＜0.05).GSR高表达的肿瘤样本富集了与氧化磷酸化、核苷酸代谢、蛋白进入线粒体过程、有丝分裂周期调控等生物学通路的基因.结论 GSR可以作为潜在的判断胃癌患者预后的指标及治疗胃癌的靶点.

abstractsObjective To explore the expressions of glutathione reductase in gastric cancer,to investigate the relationship between glutathione reductase (GSR) and clinical pathological characteristics of gastric cancers,to identify the role of GSR in evaluation of the prognosis of gastric cancer patients,and to investigate the role of GSR in the development of gastric cancer.Methods The gastric cancer datasets were searched and downloaded from The Cancer Genome Atlas (TCGA),and chip data were analyzed with clinical information.Gene set enrichment analysis (GSEA) was conducted to explore the gene sets enriched in samples with high GSR expression.Results The expression of GSR was down-regulated in high grade tumors (P ＜ 0.01).No significant difference was found between different age,Shortest tumor diameter,American Joint Committee on Cancer (AJCC) M stage,Barrett's esophagus,family history of gastric cancer,and Helicobacter pylori (H.pylori) infection.Higher expression of GSR indicated poor prognosis in gastric cancer.GSEA indicated that GSR regulates gene sets associated with oxidative phosphorylation,metabolism of nucleotides,mitochondrial protein import,and mitotic G1 S phases.Conclusions GSR can be used as an indicator to predict the prognosis of gastric cancer patients and a target for the treatment of gastric cancer.