摘要目的 评价p53自身抗体和Bmi-1自身抗体联合检测对肺癌的诊断效能.方法 采用酶联免疫吸附试验检测92例肺癌患者和80例正常对照者血清中p53、Bmi-1自身抗体的表达水平,应用受试者工作特征曲线(ROC)分析两种自身抗体的联合诊断效能.结果 肺癌患者血清p53、Bmi-1自身抗体的水平均高于正常对照,差异有统计学意义[(0.438±0.705) vs (0.045±0.035),Z=-7.667,P＜0.01;(0.117土0.061)vs (0.068±0.031),Z=-7.179,P＜0.01].血清p53、Bmi-1自身抗体在肺癌患者中的表达水平与患者的性别、年龄、病理类型、淋巴结转移情况和TNM分期等因素无关(均P＞0.05).两种自身抗体联合检测的诊断敏感度为63.0％,特异度为91.2％,ROC曲线下面积为0.881,诊断效能优于单一自身抗体.结论 血清p53、Bmi-1自身抗体联合检测具有较高的诊断价值,可能有助于肺癌的辅助诊断.

abstractsObjective To evaluate the diagnostic value of combined detection of serum autoantibodies against against p53 and Bmi-1 in lung cancer (LC).Methods Serum levels of autoantibodies against p53 and Bmi-1 were measured by enzyme-linked immunosorbent assay (ELISA) in 92 patients with LC and 80 normal controls.The combined diagnostic value was evaluated with the receiver operating characteristic (ROC) curve.Results The serum levels of autoantibodies against p53 and Bmi-1 were significantly higher in LC than in normal controls (0.438 ±0.705 vs 0.045 ±0.035,Z =-7.667,P ＜0.01;0.117±0.061 vs 0.068 ±0.031,Z =-7.179,P ＜0.01).The levels of autoantibodies against p53 and Bmi-1 were not related to age,gender,pathological classification,lymph node status or tumor-node-metastasis (TNM) stage (P ＞ 0.05).The combined detection of two autoantibodies provided an enhanced sensitivity of 63.0％,a specificity of 91.2％ and an area under curve (AUC) of 0.881,which showed better diagnostic efficiency compared to individual autoantibodies.Conclusions Combined detection of autoantibodies against p53 and Bmi-1 shows good diagnostic value,which may aid diagnosis of LC.