摘要目的 探讨miR-195及NLRX1在负压治疗后创面肉芽组织中的表达及其与创面血管生成的关系.方法 收集6例经创面负压治疗前后的难愈性创面患者肉芽组织,检测其组织中miR-195、NLRX1 mRNA表达水平,NLRX1蛋白表达水平;同时进行免疫组化(IHC)检测其组织中NLRX1的表达及CD31标记的组织微血管密度(MVD).结果在负压治疗后7d的肉芽组织中miR-195、MVD均显著高于治疗前(P＜0.05),而NLRX1则明显低于治疗前(P＜0.05);肉芽组织中miR-195与NLRX1的表达呈负相关(r=-0.856,P＜0.001);肉芽组织中NLRX1的表达与MVD呈负相关(r=-0.618,P＜0.05);肉芽组织中miR-195的表达与MVD呈正相关(r=0.630,P＜0.05).结论 负压创面治疗能促进肉芽血管生成,促进创面愈合;负压创面治疗可能通过调节miR-195的表达上调,抑制NLRX1的表达促进血管生成.

abstractsObjective To investigate the expression and the relationship with angiogenesis of miR-195 and NLR family member X1 (NLRX1) in granulation tissue after negative-pressure wound treatment (NPWT).Methods Six patients were collected who received negative pressure treatment with refractory wound granulation.The levels of miR-195, NLRX1 mRNA and NLRX1 proteins were measured.The expression of NLRX1 and the micro-vascular density (MVD) of CD31 were detected by immunohistochemistry (IHC).Results MiR-195 and MVD were significantly higher in granulation tissue after 7 days negative pressure treatment (P＜0.05), and NLRX1 was significantly lower (P ＜0.05).In granulation tissue,the expression of miR-195 was negatively correlated with NLRX1 (r =-0.856, P ＜0.001), the expression of NLRX1 was negatively correlated with MVD (r =-0.618, P ＜0.05), and the expression of miR-195 was positively correlated with MVD (r =0.630, P ＜ 0.05).Conclusions Negative pressure wound therapy can promote the formation of granulation vessels and the wound healing.The therapeutic mechanism may inhibit the expression of NLRX1 and upregulate the expression of miR-195 to promote angiogenesis.