摘要目的 探讨采用低频脉冲超声联合微泡治疗是否可以增加化疗药物——盐酸阿霉素在肿瘤组织中的转运效率.方法 24只新西兰荷瘤兔被随机分成3组,即超声微泡阿霉素组(Ad-US-MB)8只、阿霉素组(Ad)8只以及空白对照组8只.Ad-US-MB组予缓慢静脉注射盐酸阿霉素化疗后,即刻予超声微泡治疗10 min;Ad组予缓慢静脉注射盐酸阿霉素化疗后,即刻予相同剂量的生理盐水注射并超声假照10 min;空白对照组予缓慢静脉注射等剂量生理盐水后,即刻予相同剂量的生理盐水注射并超声假照10 min.观察阿霉素及肿瘤微血管的空间分布,定量对比两组阿霉素的浓度的变化.结果 Ad-US-MB组的盐酸阿霉素浓度较Ad组增加[(0.682±0.000 1)μg/ml vs (0.422±0.357) μg/ml](P＜0.05).对比观察两组的免疫荧光分布,在Ad-US-MB组中,阿霉素密集的分布在肿瘤微血管周围,甚至更远的区域;而Ad组中,在肿瘤微血管周围仅见稀疏分布的阿霉素.空白组未注射阿霉素故无阿霉素的浓度及分布.结论 低频脉冲超声联合微泡可以增加盐酸阿霉素在肿瘤组织中的转运效率.

abstractsObjective To investigate whether the low intensity pulsed ultrasound combined the microbubbles can increase the delivery of Doxorubicin in tumor.Methods 24 tumor-bearing rabbits were divided into 3 groups randomly,namely,8 rabbits in the ultrasound microbubbles adriamycin group (Ad-US-MB),8 rabbits in the adriamycin group (Ad) and 8 rabbits in the blank control group.The animals were intravenous injected with doxorubicin hydrochloride,and then the tumor of the animals were disposed by low-intensity ulrtasound and mirobubbles for 10 minutes.Then these rabbits in each group were sacrificed immediately after treatment.To observe the spatial distribution of Adriamycin and microvessel of tumors,and to quantitatively compare the changes of Adriamycin concentration between the two groups.Results The Adriamycin concentration in Ad-US-MB group [(0.682 ±0.0001) μg/ml] was higher than that in Ad group [(0.422-± 0.357) μg/ml] (P ＜ 0.05).The distribution of immunofluorescence in Ad-US-MB group was observed.Adriamycin was densely distributed around the microvessels of tumors,even farther away.In Ad group,only sparsely distributed adriamycin was found around the microvessels of tumors.The concentration distribution of adriamycin was not found in the blank control group without Doxorubicin injection.Conclusions The pulsed low frequency ultrasound combined with microbubbles can increase the permeability of chemotherapy drug.