摘要目的 探讨不同TNM分期胃癌患者磷酸化蛋白激酶B(PKB/AKT)、脾酪氨酸蛋白激酶(Syk)的表达情况.方法 选取2015年1月至2018年4月本院82例经胃镜检查及手术病理确诊胃癌患者,均取癌组织,并从中随机抽取30例患者于癌旁5 cm处取正常胃黏膜组织,免疫组织化学法检测PKB/AKT、Syk的表达情况.对比不同TNM分期胃癌患者PKB/AKT、Syk在胃癌及癌旁正常组织中的表达情况,分析胃癌组织中PKB/AKT、Syk表达与临床病理特征的相关性.结果 胃癌组织中PKB/AKT阳性表达率较癌旁组织高,Syk阳性表达率较癌旁组织低(P＜0.05);胃癌组织中PKB/AKT、Syk表达与组织学分级、肿瘤浸润、TNM分期、淋巴结转移及远处转移有关(P＜0.05),且随胃癌患者TNM分期增加,PKB/AKT阳性表达明显增加,Syk阳性表达明显下降(P＜0.05).结论 PKB/AKT与胃癌患者TNM分期呈正相关,Syk与胃癌患者TNM分期呈负相关,临床可通过检测PKB/AKT、Syk表达情况,判定胃癌TNM分期,为肿瘤治疗提供有力依据,在临床治疗中具有重要意义.

abstractsObjective To investigate the expression of phosphorylated protein kinase B (PKB/AKT) and spleen tyrosine protein kinase (Syk) in different tumor node metastasis (TNM) stages of gastric cancer patients.Methods From January 2015 to April 2018,82 patients with gastric cancer confirmed by gastroscopy and surgical pathology were enrolled in this study.All patients were selected for cancer tissue,and 30 patients were randomly selected from normal gastric mucosa at 5 cm adjacent to the tumor.Immunohistochemistry was used to detect the expression of PKB/AKT and Syk protein.To compare the expression of PKB/AKT and Syk in gastric cancer and adjacent normal tissues,and to analyze the relationship between PKB/AKT and Syk expression and clinicopathological features in gastric cancer tissues,and the correlation between PKB/AKT and Syk and TNM staging of gastric cancer patients.Results The positive expression rate of PKB/AKT in gastric cancer tissues was higher than that in adjacent tissues (P ＜ 0.05).The positive expression rate of Syk was lower than that in adjacent tissues (P ＜ 0.05).PKB/Akt and Syk gene expression in gastric cancer were related to histological grade,tumor infiltration,TNM staging,lymph node metastasis and distant metastasis (P ＜ 0.05),and the expression of PKB/AKT was significantly increased with the increase of TNM staging in gastric cancer patients,and the positive expression of Syk was significantly decreased (P ＜ 0.05).Conclusions PKB/AKT is positively correlated with TNM staging of gastric cancer patients.Syk is negatively correlated with TNM staging of gastric cancer patients.The clinical expression of PKB/AKT and Syk can be used to determine the TNM staging of gastric cancer,which provides a strong basis for tumor treatment.It is of great significance in treatment.