摘要目的:探讨氧化低密度脂蛋白受体1(OLR1)在胃癌组织中的表达及临床意义。方法:从GEPIA数据库及Kaplan-Meier Plotter在线数据库中分析OLR1在胃癌组织及癌旁组织中的表达情况及与胃癌分期、临床预后等关系；取2018年10月至12月期间在本院行胃癌根治术患者新鲜胃癌组织及癌旁组织标本6例，实时荧光定量PCR(qRT-PCR)初步验证在胃癌与癌旁组织中OLR1表达情况；取2012年1月至2014年1月期间在本院行胃癌根治术患者病理切片标本36例，免疫组化验证胃癌与癌旁组织中OLR1蛋白表达水平，分析其表达水平与患者临床病理分期相关性及预后相关性。结果:GEPIA数据库及Kaplan-Meier Plotter在线数据库分析显示，与正常组织相比，OLR1 mRNA在多种癌症组织中高表达，在胃癌组织中显著高表达( P<0.05)；OLR1 mRNA的高表达与胃癌临床分期及不良预后相关；qRT-PCR结果显示，OLR1 mRNA在胃癌组织中高表达；免疫组化结果显示，胃癌组织中OLR1的染色评分显著高于癌旁组织( P<0.001)，并且其表达水平与患者肿瘤浸润深度、肿瘤分期相关(均 P<0.05)；Kaplan-Meier生存分析显示，OLR1表达水平与胃癌患者不良预后相关，高表达组术后中位生存期为18个月，低表达组术后中位生存期为30个月，两者差异具有统计学意义( P＝0.016)。 结论:OLR1在胃癌组织中高表达，其表达水平与患者肿瘤进展及不良预后相关。

abstractsObjective:To investigate the expression and clinical significance of oxidized low density lipoprotein receptor 1 (OLR1) in gastric cancer.Methods:In gastric cancer tissues and adjacent tissues, the relationship between the expression of OLR1 and the stage and clinical prognosis of gastric cancer was analyzed from the Gene Expression Profiling Interactive Analysis (GEPIA) database and Kaplan-Meier Plotter online database. We collected 6 case of fresh gastric cancer tissues and adjacent tissues of patients undergoing radical gastrectomy in our hospital from October 2018 to December 2018, The extracted RNA were analyzed by quantitative real-time polymerase chain reaction (qRT-PCR) to verify the expression in gastric cancer and adjacent tissues. From January 2012 to January 2014, 36 patients with pathological biopsy specimens of radical gastrectomy in our hospital were examined. Immunohistochemistry was used to confirm the correlation between protein expression level of OLR1 in gastric cancer and paracancerous tissues and clinical pathological stage and prognosis.Results:GEPIA database and Kaplan-Meier Plotter online database analysis showed that OLR1 mRNA was highly expressed in various cancer tissues and significantly higher in gastric cancer tissues than normal tissues ( P<0.05). High expression of OLR1 mRNA is associated with clinical stage and poor prognosis of gastric cancer; qRT-PCR results showed that OLR1 mRNA was highly expressed in gastric cancer tissues; the results of immunohistochemistry showed that the staining score of OLR1 in gastric cancer tissues was significantly higher than that in adjacent tissues ( P<0.001), and its expression level was correlated with depth of tumor invasion and clinical tumor node metastasis (TNM) stage (both P<0.05). Kaplan-Meier survival analysis showed that the expression level of OLR1 was associated with poor prognosis in patients. The median survival time was 18 months in OLR1 high expression group and 30 months in OLR1 low expression group, with statistically significant difference ( P=0.016). Conclusions:OLR1 is highly expressed in gastric cancer tissues, and its expression level is associated with tumor progression and poor prognosis.