摘要目的 探讨气管内给予抗肿瘤坏死因子抗体(TNF-αAb)对体外循环(CPB)肺损伤组织超微结构的影响.方法 选取健康家兔28只,随机分成4组,每组7只,即:Ⅰ组为单纯开胸组;Ⅱ组为单纯CPB组;Ⅲ组为CPB+经气管插管注入生理盐水组;Ⅳ组为CPB+TNF-αt Ab组.给药方法 是于术前和主动脉开放后经气管插管注入抗兔TNF-α Ab(总量2400pg/kg).采取肺组织样本,电镜观察其超微结构,并对TNF-α mRNA和TNF-α蛋白的表达及细胞凋亡情况进行观察.结果 TNF-α Ab明显抑制了肺源性TNF-α的释放.降低了肺含水量,减少了肺组织细胞凋亡的发生,且病理形态学改变较轻.结论 气管内应用TNF-α Ab能明显减轻体外循环时肺组织超微结构的损伤.

abstractsObjective To study the effect of intratracheal anti-tumor necrosis factor-α antibody(TNF-αAb)on ultra-structure of lung after cardiopulmonary bypass(CPB).Method 28 healthy rabbits were selected and randomly evenly divided into four groups:I group only received open chest operation;Ⅱ-Ⅳ groups underwent CPB.In the IV group,rabbit TNF-α Ab (2400 ps/kg)Was dropped into the intracheal tube before operation and just after releasing the aortic clamp.Saline was given to the Ⅲ group by the same way.Water volume,TNF-α mRNA,TNF-α protein,apoptosis and pathomorphological changes were measured in the lung tissues.Results TNF-α Ab can re-duce releasing of TNF-α.It could also reduce the occurrence of apeptosis and attenuate pathomorphological changes in the lung tissue.Conclusion Intratracheal TNF-α Ab markedly lessenes the injury of nltrastructure of lung after CPB.