摘要目的 探讨血管紧张素Ⅱ1型受体(AT1R)基因多态性与原发性高血压(EH)之间的关系.方法 应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)对200例汉族EH患者(EH组)和192例正常血压者(对照组)的AT1R基因1166A/C及-810A/T多态性进行检测,测定空腹血糖、甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)及高密度脂蛋白胆固醇(HDL-C)等生物化学指标,分析各基因型和等位基因频率与EH的关系.结果 1166A/C等位基因和基因型频率在EH组和对照组的分布无统计学差异(P均＞0.05),-810A/T各基因型在EH组和对照组间差异有统计学意义(x2=10.862,P=0.004),-810T等位基因频率在EH组显著增高[22.5%(102/400)与11.5%(44/384),x2=12.745,P=0.000],用Logistic回归模型校正了传统危险因素的影响后,-810AT和TT基因型的携带者患高血压的危险性显著增加(P=0.003,OR值为2.57,95%CI:1.37～4.84).结论 AT1R-810A/T多态性与EH发病相关,-810T等位基因可能是EH发病的风险因子.

abstractsObjective To investigate whether angiotensin Ⅱ type 1 receptor(AT1R)gene polymorphism is associated with essential hypertension(EH). Methods A total of 200 hypertension patients and 192 normotensive controls were enrolled. The AT1R gene 1166A/C and -810A/T polymorphism were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-PFLP), and the association between the SNPs and the EH were analyzed statistically. Some biochemical index such as serum glucose (GLU) and total cholesterol (TC),triglyceride (TG), high density lipoprotein (HDL-C) and low density lipoprotein (LDL-C) were also measured. Results There was no significant difference between two groups of 1166A/C polymorphisms of AT1R gene(P ＞ 0.05 ). However, for the -810A/T polymorphism of AT1R gene, -810 AT and TT genotypes frequencies were significantly higher in EH patients than control (P = 0. 004). The -810T allele frequencies were higher in case than in control (22.5% vs. 11.5% ;P =0.000). We also found an association between EH and -810AT and TT genotypes by logistic regression analysis ( P = 0. 003 ), adjusted for other risk factors. The odds ratio was 2.57 (95% CI:1. 37 ～4. 84). Conclusions AT1R -810A/T polymorphism is associated with EH and -810T allele may be a risk factor of hypertension