摘要目的：探讨多发性骨髓瘤（ MM）患者骨髓单个核细胞泛素连接酶CUL4B mRNA的表达和血清IL?6水平变化的临床意义。方法采用实时荧光PCR相对定量法及酶联免疫吸附法检测46例MM患者和40例非血液肿瘤患者骨髓单个核细胞中CUL4B基因及血清IL?6的表达情况。结果 MM组CUL4B mRNA相对表达量为3．455（2．098，4．768），MM组IL?6高于对照组[23．985（23．015，26．878）、6．205（5．405，10．215） ng／L]，差异有统计学意义（Z＝－8．262，P＝0．000），临床分期为Ⅰ、Ⅱ、Ⅲ期MM患者 CUL4B mRNA 表达水平分别为2．424（1．881，3．583）、3．594（2．093，5．738）、4．300（2．928，7．272），IL?6分别为23．115（22．723，23．568）、23．630（22．860，26．625）、26．350（24．995，30．550） ng／L，随着临床分期增加，CUL4B mRNA和IL?6水平呈递增趋势（ P均＜0．05）。 MM患者CUL4B mRNA表达水平治疗后低于治疗前[1．665（1．420，2．298）、3．455（2．098，4．768） ng／L ]，差异有统计学意义（ Z＝－4．955，P＝0．000）， IL?6治疗后低于治疗前[15．160（11．705，17．195）、23．985（23．015，26．878） ng／L]，差异有统计学意义（ Z＝－7．981，P＝0．000）。 MM患者CUL4B与IL?6相关（ r＝0．386，P＝0．008）。结论检测CUL4B和IL?6对MM 患者的临床分期、病情判断和疗效观察具有重要意义。

abstractsObjective To investigate the clinical significance of CUL4B and interleukin?6( IL?6) levels in patients with multiple myeloma(MM).Methods The mRNA of CUL4B and IL?6 were detected by real?time relative quantitative PCR and enzyme?linked immunosorbent assay( ELISA) in 46 cases of MM and 40 cases of non hematologic cancer patients.Results CUL4B concentrations in patients with MM were 3.455(2.098,4. 768).IL?6 concentrations in patients with MM were significantly higher than those of the normal control group ((23. 985(23. 015,26. 878) ng/L vs. 6. 205(5. 405,10. 215) ng/L,Z=-8. 262,P=0. 000). The expression of CUL48 mRNA in Stage Ⅰ,Ⅱ,Ⅲ was 2. 424 ( 1. 881, 3. 583 ) , 3. 594 ( 2. 093, 5. 738 ) , 4. 300 ( 2. 928, 7. 272) respectively, and IL?6 was 23. 115 ( 22. 723, 23. 568 ) , 23. 630 ( 22. 860, 26. 625 ) , 26. 35 ( 24. 995, 30. 550) ng/L respectively,with the increase of clinical stage,the level of CUL4B and IL?6 showed increasing trend( P<0. 05) . CUL4B concentrations were significantly decreased in MM patients after treatment than before treatment(1. 665(1. 420,2. 298) vs. 3. 455(2. 098,4. 768),Z=-4. 955,P=0. 000). IL?6 concentrations were significantly decreased in MM patients after treatment than before treatment( 15. 160( 11. 705,17. 195) ng/L vs. 23. 985(23. 015,26. 878) ng/L,Z=-7. 981,P=0. 000). CUL4B level in patients was positively correlated with IL?6( r=0. 386,P=0. 008 ) . Conclusion It is of great significance to detect CUL4B and IL?6 in patients with MM on clinical stage,the condition of judgment and efficacy observation.