摘要目的：观察整合素β4（ integrinβ4）在哮喘患者急性发作期及缓解期的表达及其与Th1／Th2细胞因子间的相互关系，探讨integrinβ4与哮喘患者免疫功能之间的关系。方法收集深圳市第七人民医院2013年5月至2015年6月门诊或住院的哮喘患者35例，采用ELISA和Western?blotting法对哮喘急性发作期、缓解期患者血清中IL?4、干扰素γ（ IFN?γ）和integrinβ4蛋白进行检测，分析比较哮喘患者急性发作期及缓解期IL?4、IFN?γ、integrinβ4蛋白的水平。结果哮喘缓解期患者血清integrinβ4蛋白表达水平（0．633±0．032）较急性发作期（0．375±0．107）明显升高，两者比较差异有统计学意义（t＝3．31， P＜0．01）。哮喘缓解期IFN?γ水平[（49．3±6．4） g／L]较哮喘急性发作期[（16．7±7．2） g／L]明显升高，两者比较差异有统计学意义（t＝3．11，P＜0．05），而血清IL?4分别为（25．3±3．6） ng／L 与（43．7±11．2） ng／L，两者比较差异有统计学意义（ t＝3．23，P＜0．05）。 integrinβ4蛋白表达水平与IL?4／IFN?γ比值呈直线负相关（哮喘急性发作期：r＝－0．749，P＝0．05；哮喘缓解期：r＝－0．745，P＝0．015）。结论血清integrinβ4水平和Th1／Th2细胞因子间存在负相关，和哮喘患者免疫功能密切相关，integrinβ4可能参与哮喘患者气道炎症的发生与发展。

abstractsObjective To detect the changes of serum integrinβ4 level during both exacerbation and clinical remission of asthmatic patients, and observe the correlation between the changes and balance of Th1/Th2,and to explore the relationship between integrinβ4 and the immune function in asthmatic patients.Methods Thirty?five cases patients who were hospitalized in the Seventh People's Hospital of Shenzhen from May 2013 to June 2015 were collected into this study.Serum interleukin?4(IL?4),interferonγ(IFN?γ),and integrinβ4 were measured with ELISA and western?blotting methods during both exacerbation and remission of asthmatic patients. Then the level of IL?4, IFN?γand integrinβ4 of the two groups were compared. Results The level of serum integrinβ4 protein in the remission stage of asthma was significantly higher than that in the acute attack period, and the difference was statistically significant(0.633±0.032 vs. 0.375±0.107,t=3.31,P<0.01).The level of ser?um IFN?γ in the remission stage of asthma was significantly higher than that in the acute attack period,and the difference was statistically significant((49.3±6.4) g/L vs. (16.7±7.2) g/L,t=3.11,P<0.05),and the level of IL?4 was (25.3±3.6) ng/L and (43.7±11.2) ng/L respectively,the difference between the two groups had sta?tistical significance(t=3.23,P<0.05).The serum level of integrinβ4 and IL?4/IFN?γratio were passively corre?lated(the acute attack period:r=0.749,P=0.05;the remission stage of asthma:r=0.745,P=0.015).Conclu?sion The serum level of integrinβ4 is passively related to IL?4/IFN?γ. Integrinβ4 may play an important role between immune function and the development in airway inflammation of asthmatic patients.