摘要目的:探讨腹腔镜下水分离剥除联合缝合止血法对双侧卵巢子宫内膜异位囊肿(ovarian endometriotic cyst，OEC)剥除术后卵巢储备功能的保护作用。方法:选取2016年1月至2018年1月上海市嘉定区中医医院行腹腔镜下双侧OEC剥除术的患者60例，根据随机数字表法分为研究组(水分离剥除联合缝合止血组)和对照组(直接剥除联合电凝止血组)各30例，比较两组患者手术时间、手术前后血红蛋白水平的变化及术前和术后1、3、6个月基础状态的卵泡刺激素(follicle stimulating hormone，FSH)、黄体生成素(luteinizing hormone，LH)、雌二醇(estradiol，E2)及抗苗勒管激素(anti-Mullerian hormone，AMH)水平。结果:研究组手术时间为(47.52±10.11) min、手术前后血红蛋白下降量为(0.55±0.26) g/L 、住院时间为(6.1±0.3) d，对照组分别为(48.01±10.24) min、(0.56±0.25) g/L 、(6.2±0.4) d，两组比较差异均无统计学意义( t值分别为0.056、0.964、0.863， P均>0.05)。研究组手术前FSH、E2、LH及AMH水平分别为(6.15±2.31) U/L、(152.41±41.40) nmol/L、(5.44±1.52) U/L、(2.21±0.13) μg/L；术后1个月分别为(6.21±2.24) U/L、(150.63±40.33) nmol/L、(5.13±1.58) U/L、(2.18±0.16) μg/L；术后3个月分别为(6.52±2.41) U/L、(149.57±42.37) nmol/L、(5.30±1.45) U/L、(2.17±0.15) μg/L；术后6个月分别为(6.53±2.44) U/L、(151.36±41.54) nmol/L、(4.98±1.61) U/L、(2.20±0.08) μg/L。对照组患者手术前FSH、E2、LH及AMH水平分别为(6.14±2.21) U/L、(153.31±40.39) nmol/L、(5.51±1.46) U/L、(2.23±0.13) μg/L；术后1个月分别为(8.11±2.44) U/L、(131.43±41.23) nmol/L、(5.92±1.64) U/L、(1.58±0.14) μg/L；术后3个月分别为(8.42±2.35) U/L、(135.67±40.38) nmol/L、(6.12±1.51) U/L、(1.54±0.16) μg/L；术后6个月分别为(9.17±2.64) U/L、(133.66±40.44) nmol/L、(6.28±1.74) U/L、(1.51±0.13) μg/L；研究组患者术前FSH、E2、LH和AMH水平与术后第1、3、6个月比较差异均无统计学意义( P均>0.05)。对照组患者术前FSH水平与术后1、3、6个月比较差异均有统计学意义( P均<0.05)；对照组术前E2水平与术后1个月比较差异有统计学意义( P<0.05)；对照组术前AMH水平与术后1、3个月比较差异均有统计学意义( P均<0.05)。术后6个月研究组患者FSH、LH、E2、AMH水平均优于对照组，差异均有统计学意义( P均<0.05)。 结论:腹腔镜水分离剥除联合缝合止血法剥除双侧OEC不增加手术时间及术中出血量，不影响术后卵巢储备功能。

abstractsObjective:To investigate the protective effect of laparoscopic water separation and removal combined with suture hemostasis on ovarian reserve function after bilateral ovarian endometriotic cyst (OEC) stripping.Methods:From January 2016 to January 2018, 60 patients with bilateral ovarian endometriosis cystectomy underwent laparoscopic surgery in the Department of Obstetrics and Gynecology, Jiading Hospital of traditional Chinese medicine.According to the random number table method, they were divided into study group (water separation and stripping combined suture hemostasis group) and the control group (direct stripping combined with electrocoagulation hemostasis group), 30 cases in each group.The changes of operation time, hemoglobin level before and after operation and the levels of follicle stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E2) and anti Mullerian hormone (AMH) were compared between the two groups.Results:There was no significant difference in operation time (47.52 ±10.11) min, hemoglobin decrease (0.55 ±0.26) g/L, hospital stay (6.1 ±0.3) d, control group (48.01 ±10.24) min, hemoglobin decrease (0.56 ±0.25) g/L and hospitalization time (6.2 ±0.4) d before and after operation ( t=0.056, 0.964, 0.863, all P>0.05). The levels of FSH, E2, LH and AMH in the study group before operation were (6.15 ±2.31) U/L, (152.41 ±41.40) nmol/L, (5.44 ±1.52) U/L and (2.21 ±0.13) μg/L, respectively.One month after operation, they were(6.21±2.24) U/L, (150.63±40.33) nmol/L, (5.13±1.58) U/L, (2.18±0.16) μg/L, respectively.Three months after operation, they were (6.52±2.41) U/L, (149.57±42.37) nmol/L, (5.30±1.45) U/L, (2.17± 0.15) μg/L, respectively.Six months after operation, they were (6.53±2.44) U/L, (151.36±41.54) nmol/L, (4.98±1.61) U/L, (2.20±0.08) μg/L, respectively.The levels of FSH, E2, LH and AMH in the control group before operation were (6.14±2.21) U/L, (153.31±40.39) nmol/L, (5.51±1.46) U/L, (2.23±0.13) μg/L, respectively.One month after operation, they were (8.11±2.44) U/L, (131.43±41.23) nmol/L, (5.92±1.64) U/L, (1.58±0.14) μg/L, respectively.Three months after operation, they were (8.42±2.35) U/L, (135.67±40.38) nmol/L, (6.12±1.51) U/L, (1.54±0.16) μg/L, respectively.Six months after operation, they were (9.17±2.64) U/L, (133.66±40.44) nmol/L, (6.28±1.74) U/L, (1.51±0.13) μg/L, respectively.There was no significant difference in the levels of FSH, E2, LH and AMH between the two groups (all P>0.05). There was significant difference between the preoperative FSH level and the postoperative 1, 3, 6 months in the control group (all P<0.05); there was significant difference between the preoperative E2 level and the postoperative 1 month in the control group ( P<0.05); there was statistical significance between the preoperative AMH level and the postoperative 1, 3 months in the control group (all P<0.05). The levels of FSH, LH, E2 and AMH in the study group were significantly higher than those in the control group (all P<0.05). Conclusion:Laparoscopic hydrodissection combined with suture hemostasis does not increase the operation time and the amount of bleeding, and does not affect the ovarian reserve function after operation.