摘要目的:探讨高迁移率核小体结合蛋白5（high mobility group nucleosome-binding protein，HMGN5）在肝癌组织及肝癌HepG2细胞中的表达及对肝癌HepG2细胞生长的影响。方法:选取潍坊市阳光融和医院2017年1月至2020年6月行手术切除并经术后病理确诊的肝细胞癌患者70例，收集其临床和病理资料，以及手术切除的肝癌组织和癌旁正常肝组织，记录术后生存时间。应用免疫组织化学方法检测肝癌组织和癌旁正常肝组织中HMGN5蛋白的表达情况，比较HMGN5蛋白的表达情况，以及不同临床特征肝细胞癌患者肝癌组织中HMGN5蛋白的阳性表达率，分析HMGN5表达与肝细胞癌患者预后的关系。应用实时荧光定量聚合酶链反应法检测肝癌HepG2细胞和肝HL-7702细胞中HMGN5 mRNA的表达情况。用HMGN5短发夹RNA转染HepG2细胞后，通过细胞增殖实验评估敲除 HMGN5基因对肝癌HepG2细胞增殖的抑制作用，使用流式细胞仪分析敲除 HMGN5基因对肝癌HepG2细胞凋亡情况的影响。呈正态分布的计量资料以 xˉ± s表示，组间比较采用两独立样本 t检验；计数资料以例（%）表示，组间比较采用 χ2检验；患者生存分析采用Kaplan-Meier法。 结果:免疫组织化学染色结果显示，HMGN5在肝癌组织中的染色很强，而在癌旁正常肝组织中的染色很弱。70例肝细胞癌患者手术切除的肝癌组织中HMGN5蛋白表达阳性48例，病理分级3级+4级以及临床分期Ⅲ期+Ⅳ期肝细胞癌患者肝癌组织中HMGN5蛋白阳性表达率分别高于1级+2级、Ⅰ期+Ⅱ期患者［80.0%（28/35）比57.1%（20/35），80.6%（29/36）比55.9%（19/34）］，差异均有统计学意义（ χ2值分别为4.24、4.94， P值分别为0.039、0.026）。HMGN5阳性表达组患者的累积生存率与阴性表达组比较差异无统计学意义（ χ2=3.81， P=0.051）。肝癌HepG2细胞中HMGN5 mRNA的表达高于正常人肝HL-7702细胞［（4.51±0.45）比（1.35±0.27）］，差异有统计学意义（ t=10.43， P<0.001）。 HMGN5基因被敲除24、48、72 h后，肝癌HepG2细胞的生存率均低于肝HL-7702细胞［（71.6±0.8）%比（97.6±1.4）%，（56.3±1.0）%比（96.7±1.5）%，（46.7±1.1）%比（96.1±1.2）%］，差异均有统计学意义（ t值分别为-28.07、-38.74、-52.29，均 P<0.001）。敲除 HMGN5基因后，肝癌HepG2细胞的凋亡率高于肝HL-7702细胞［（18.63±1.76）%比（1.81±0.21）%］，差异有统计学意义（ t=16.44， P<0.001）。 结论:HMGN5在肝癌组织及肝癌HepG2细胞中高表达，在肝细胞癌生长过程中发挥着重要作用，可作为肝细胞癌治疗的潜在靶点。

abstractsObjective:To investigate the affection of high mobility group nucleosome-binding protein 5(HMGN5) gene in the hepatocellular cancer tissue and HepG2 cell and its function in the growth of HepG2 cell.Methods:70 Patients with hepatocellular carcinoma who under-went surgical resection and were confirmed by postoperative pathology in Sunshine Union Hospital of Weifang City from January 2017 to June 2020 were selected. Their clinical and pathological data, surgical resection of liver cancer tissue and adjacent normal liver tissue were collected, their survival time were also recorded. The contents of HMGN5 protein in the hepatocellular cancer tissues and adjacent normal liver tissues of 70 patients were detected by immunohistochemistry. Compare the expression of HMGN5 protein in liver cancer tissue and normal liver tissue and the positive expression rate of HMGN5 protein in liver cancer tissues of hepatocellular carcinoma patients with different clinical characteristics, to analyze the relationship between HMGN5 expression and prognosis of patients with hepatocellular carcinoma.The mRNA contents of HMGN5 gene in HepG2 cells and HL-7702 cells were determined by quantitative real time polymerase chain reaction(qRT-PCR) method. After transfectHepG2 cells with HMGN5 shRNA, the proliferation ability of HepG2 cells were evaluated by cell proliferation assay and the cell apoptosis was analyzed by flow cytometry. Measurement data with normal distribution were expressed as ±s, two independent samples t test was used for comparison between groups. Counting data was expressed as n(%), χ2 test was used for comparison between groups. Survival analysis of patients was performed by Kaplan-Meier method. Results:Immunohistochemical tests showed that HMGN5 staining is strong in liver cancer tissue, but weak in normal liver tissue. HMGN5 protein expression was positive in 48 of 70 patients with hepatocellular carcinoma. The positive expression rate of HMGN5 protein in hepatocellular carcinoma tissues of patients with pathological grade 3+4 and clinical stage Ⅲ+Ⅳ was higher than that of patients with grade 1+2 and stage Ⅰ+Ⅱ, respectively. There was no significant difference in cumulative survival rate between HMGN5 positive expression group and HMGN5 negative expression group ( χ2=3.81, P=0.051). The results of qRT-PCR showed that the expression level of HMGN5 mRNA in hepatoma HepG2 cells was higher than that in normal liver HL-7702 cells [(4.51±0.45) vs (1.35±0.27), the difference was statistically significant ( t=10.43, P=<0.001). After 24, 48 and 72h of the HMGN5 gene was knockout, the survival rate of HepG2 hepatoma cells was lower than that of HL-7702 hepatoma cells (all P<0.05) and the apoptosis rate of hepatoma HepG2 cells was higher than that of liver HL-7702 cells [(18.63±1.76)%vs(1.81±0.21)%] ,the difference was statistically significant ( t=16.44, P<0.001). Conclusions:HMGN5 genes and proteins are highly expressed in the hepatocellular cancer tissues and HepG2 cells. HMGN5 gene plays an important role in the growth of hepatocellular cancer and can be used as a potential target of treatment for hepatocellular cancer.