摘要Objective:To analyze the changes in peripheral natural killer T-cells (NKT) and gammadelta T-cells (γδ T-cell) in patients with minimal residual leukemia (MRL) before and after being treated with Yiqi Bushen Granule (益气补肾颗粒,YBG) in order to determine their significance in prognosis of the disease.Methods:Before and after treatment,the changes in 36 patients (16 males and 20 females) receiving long-term (more than 3 months) YBG therapy were analyzed using multi-parameter flow cytometry,with 34 healthy persons (19 males and 15 females) acting as controls.Results:The absolute value and percentage of NKT cells and γδ T-cells were all significantly raised after treatment,for NKT cells,0.52% ± 0.39% to 0.83% ± 0.66% and 7.25 ± 7.77cell/μ L to 12.86 ± 11.99 cell/μ L,for γδ T-cells,6.08% ± 3.03% to 7.24% ± 2.78% and 83.97 ± 48.09 cell/μ L to 110.53±54.12 cell/μ L,respectively (P＜0.05 or P＜0.01).Conclusion:YBG could regulate the immune function and elevate the amount of NKT cells and γδ T-cells,thus to kill or suppress the residual leukemic cell in the body,which might be one of the mechanisms of YBG in prolonging the disease-free survival in MRL patients.