摘要Objective:To observe the effects of Dachengqi Decoction (大承气汤,DCQD) on morphological changes in the network of enteric nerve-interstitial cells of Cajal (ICCs)-smooth muscle cells (SMC) of enteric deep muscular plexuses (DMP) in the rats with multiple organ dysfunction syndrome (MODS).Methods:One hundred Wistar rats of both sexes weighing 200 to 250 g were randomly divided into the control group,MODS group,and DCQD group.The morphologic changes of enteric nerve-ICC-SMC network in the DMP of intestine was observed using c-Kit and vesicular acetylcholine transporter/neuronal nitric oxide synthase immunohistochemical double-staining with whole-mount preparation technique,confocal laser scanning microscopy,and electron microscopy.Results:Compared with the control group,the distribution and densities of cholinergic/nitrergic nerves and ICC in the DMP (ICC-DMP) of intestine in the MODS group were significantly decreased (P＜0.01),and the network of cholinergic nerve-ICC-SMC was disrupted; and the ultrastructural features of ICC-DMP,enteric nerve,and SMC were severely damaged.After treatment with DCQD,the damage in the network of enteric nerve-ICC-SMC was significantly recovered.Compared with the MODS group,the distribution and densities of cholinergic/nitrergic nerves and ICC-DMP in the DCQD group were significantly increased (P＜0.01); and the ultrastructural features of ICC-DMP,enteric nerve,smooth muscle calls were significantly recovered.Cenclusions:DCQD can improve the gastrointestinal motility in MODS.The mechanism may be related to the effect of repairing the damages in the network of enteric nerve-ICC-SMC.