摘要Objective:To investigate effects of Dahuang Zhechong Pill(大黄(度)虫丸,DHZCP) on the cell cycle and the related signal pathways in vascular smooth muscle cells (VSMCs) stimulated by platelet-derived growth factor (PDGF) with the method of serum pharmacology.Methods:DNA synthesis in VSMCs was examined by detecting 5'-bromo-2'-deoxyuridine incorporation with the immunocytochemical method.The cycle of VSMCs was evaluated with flow cytometry.Expressions of cyclln D1,p27,protein kinase Cα (PKCα),and phosphorylated extracellular signal regulated kinase 1/2 (ERK1/2) were quantified by Western blot method.Results:DHZCP containing serum significantly inhibited DNA synthesis of PDGF-stimulatod VSMCs,arrestedthe cells in G1 phase,modulated the protein expressions of cyclin D~ and p27,and suppressed the activation of PKC α and ERK1/2.Conclusion:DHZCP containing serum inhibits VSMCs proliferation via modulating the expressions of cell cycle proteins to arrest the cell in G1 phase,which is attributed to,at least in part,suppressing PKC α-ERK1/2 signaling in VSMCs.