摘要Objective:To investigate the protective effects of the natural medicinal monomer isopsoralen(ISR)with estrogenic activity against oxidative damage in human lens epithelial cells B3(HLE-B3)caused by hydrogen peroxide(H2O2)and to pursue the possible mitochondrial proteomic regularity of the protective effects.Methods:HLE-B3 cells were treated with H2O2(300 μmol/L),β-estradiol(E2:10-8 mol/L)and H2O2,ISR(10-5 mol/L)and H2O2,or left untreated.Altered expressions of all mitochondrial proteins were analyzed by protein array and surface-enhanced laser desorption ionization time of flight mass spectrometry(SELDI-TOF-MS).The mass/charge(m/z)ratios of each peak were tested by the Kruskal-Wallis rank sum test,and the protein peak value of the m/z ratio for each treatment by pair comparison was analyzed with the Nemenyi test.Results:H2O2 up-regulated the expressions of two protein spots(with m/z of 6532 and 6809).E2 mitigated the oxidative damage,and the expression of one protein spot(m/z 6532)was down-regulated.In contrast,ISR down-regulated both of protein spots(m/z 6532 and 6809).Conclusions:ISR could effectively inhibit H2O2-induced oxidative damage in HLE-B3 Cells.The protein spot at m/z of 6532 might be the target spot of ISR against oxidative damage induced by H2O2.