摘要Objective:To investigate the anti-tumor effects of Pien Tze Huang(PZH)in mouse models of B16-F10 melanoma,MC38 colorectal cancer,Hep1-6 hepatocellular carcinoma and chemically induced hepatocellular carcinoma model.Methods:Various tumor models,including B16-F10,MC38 and Hep1-6 tumor hypodermic inoculation models,B16-F10 and Hep1-6 pulmonary metastasis models,Hep1-6 orthotopic implantation model,and chemically induced hepatocellular carcinoma model,were utilized to evaluate the anti-tumor function of PZH.Tumor growth was assessed by measuring tumor size and weight of solid tumors isolated from C57BU6 mice.For cell proliferation and death of tumor cells in vitro,as well as T cell activation markers,cytokine production and immune checkpoints analysis,single-cell suspensions were prepared from mouse spleen,lymph nodes,and tumors after PZH treatment.Results:PZH demonstrated significant therapeutic efficacy in inhibiting tumor growth(P＜0.01).Treatment with PZH resulted in a reduction in tumor size in subcutaneous MC38 colon adenocarcinoma and B16-F10 melanoma models,and decreased pulmonary metastasis of B16-F10 melanoma and Hep1-6 hepatoma(P＜0.01).However,in vitro experiments showed that PZH only had slight impact on the cell proliferation and survival of tumor cells(P＞0.05).Nevertheless,PZH exhibited a remarkable ability to enhance T cell activation and the production of interferon gamma,tumor necrosis factor alpha,and interleukin 2 in CD4+T cells in vitro(P＜0.01 or P＜0.05).Importantly,PZH substantially inhibited T cell exhaustion and boosted cytokine production by tumor-infiltrating CD8+T cells(P＜0.01 or P＜0.05).Conclusion:This study has confirmed a novel immunomodulatory function of PZH in T cell-mediated anti-tumor immunity,indicating that PZH holds promise as a potential therapeutic agent for cancer treatment.