摘要Objectives:To explore the effect of Pollen Pini(PP)on SMMC-7721 hepatoma cells.Methods:The anti-proliferative effects of PP on SMMC-7721 cells were evaluated using cell counting kit-8 assay following 48 h treatment with concentrations ranging from 1.25 to 37.50 μg/μL.Flow cytometry analysis at the half maximal inhibitory concentration(IC50)revealed significant apoptosis induction and cell cycle alterations.For in vivo evaluation,SMMC-7721 xenograft-bearing nude mice were administered either vehicle(0.9％NaCl)or PP(500 mg/kg)via intraperitoneal injection every other day for 3 weeks.Subsequent tumor analysis included mass spectrometry-based proteomics and examination of phosphoinositide-3 kinase(PI3K)/protein kinase B(Akt)/Myc pathway proteins by immunohistochemistry and Western blot.Combination therapy with 25 μ mol/L PI3K inhibitor and PP(ICso)showed optimal efficacy,with Western blot revealing maximal protein modulation at 24 h.Results:PP had an anti-tumor effect on SMMC-7721 cells in vitro,with the IC50 concentration of 18.94 mg/mL.PP could promote the death of SMMC-7721 cells(P＜0.01)and regulate the cell cycle more in G0/G1 phase cells(P＜0.01).After the treatment effect of PP,the protein content of SMMC-7721 cells changed and the contents of cancer-promoting proteins PI3K,Akt and Myc decreased(P＜0.01).PI3K inhibitor could reduce the proliferation of SMMC-7721 cells,and PI3K inhibitor combined with PP significantly reduced the expression of PI3K in SMMC-7721 cells(P＜0.01).Conclusion:PP has an anti-tumor effect on SMMC-7721 cells through the PI3K-Akt signaling pathway.