摘要目的 通过比较膜突蛋白和上皮细胞钙黏蛋白(E-cadherin)在非特殊型乳腺浸润性癌(BIC-NST)、乳腺导管原位癌(BDCIS)及癌旁正常组织中的表达,探讨膜突蛋白和E-cadherin与乳腺癌临床病理参数的相关性.方法 收集惠州市中心人民医院2008年1月至2010年12月乳腺癌病例标本,采用组织芯片免疫组织化学SP法,检测104例BIC-NST、84例BDCIS及53例癌旁组织中膜突蛋白和E-cadherin的表达;采用Western blot检测16例新鲜BIC-NST组织的膜突蛋白表达.结果 膜突蛋白在BIC-NST及BDCIS组织中的表达明显高于癌旁组织(P＜0.01),而E-cadherin在BIC-NST及BDCIS组织中的表达明显低于癌旁组织(P＜0.01);膜突蛋白在BIC-NSTⅢ级分化组的表达率明显高于Ⅰ级分化组(P＜0.05),而且组织分级越高,膜突蛋白表达越高,呈明显的正相关(P＜0.05),但E-cadherin在BIC-NSTⅢ级分化组的表达率明显低于Ⅰ级分化组(P＜0.05),而且组织分级越高,E-cadherin表达越低,呈明显的负相关(P＜0.05).在BDCIS组织中膜突蛋白及E-cadherin表达与分级不相关.膜突蛋白表达在患者临床分期Ⅲ+Ⅳ期中的表达明显高于Ⅰ+Ⅱ期的表达(P＜0.01),与淋巴结转移相关(P＜0.01),但与年龄、肿物大小及脉管浸润不相关;E-cadherin表达在患者临床分期Ⅲ+Ⅳ期中的表达明显低于Ⅰ+Ⅱ期的表达(P＜0.01),与淋巴结转移及脉管浸润相关(P＜0.01),但与年龄、肿物大小不相关;膜突蛋白及E-cadherin在BIC-NST(P=0.021)及BDCIS(P=0.032)中的表达呈负相关.结论 膜突蛋白信号的高表达及E-cadherin信号的低表达与乳腺癌的发生发展密切相关,可为乳腺癌的基因治疗提供新的靶点.

abstractsObjective To investigate the correlation of moesin and E-cadherin with biological behavior of breast cancer and its mechanism by comparing expression of moesin and E-cadherin in breast invasive carcinoma of no specific type (BIC-NST),breast ductal carcinoma in situ (BDCIS) and normal breast tissues adjacent to carcinoma.Methods Breast cancer cases of the Huizhou Municipal Center People Hospital were collected between Jan 2008 and Dec 2010,expression of moesin and E-cadherin in 104 cases of BIC-NST,84 cases of BDCIS and 53 cases of normal breast tissues adjacent to carcinoma were detected by tissue-microarray and SP immunohistochemical staining.Western blot was used to detect moesin expression of 16 BIC-NST fresh tissues.Results Expression rate of moesin in BIC-NST and BDCIS were significantly higher than normal tissues(P ＜ 0.01),but the expression rate of E-cadherin in BIC-NST and BDCIS were significantly lower than those of normal tissues(P ＜ 0.01).Expression rate of moesin in BIC-NST grade Ⅲ group was significantly higher than that of the grade Ⅰ group.There was a significantly positive correlation between histological grade and moesin expression(P ＜ 0.05).However,E-cadherin expression rate in BICNST grade Ⅲ group was significantly lower than that in grade Ⅰ group,and there was a significantly negative correlation between histological grade and E-cadherin expression (P ＜ 0.05).Moreover,no significant correlation was observed between moesin and E-cadherin expression in BDCIS tissues.Expression of moesin in clinical stage Ⅱ + Ⅲ BIC-NST was significantly higher than that in stage Ⅰ (P ＜ 0.01).Expression of moesin was significantly associated with lymph node metastasis (P ＜ 0.01).But no significant correlation was observed between moesin expression and age,tumor size and vascular invasion.However,expression of E-cadherin in clinical stage Ⅱ + Ⅲ BIC-NST was significantly lower than that in stage Ⅰ (P ＜ 0.01).Expression of E-cadherin was significantly associated with lymph node metastasis and vascular invasion (P ＜ 0.01).But no significant correlation was observed between E-cadherin expression,age and tumor size.There was a negative correlation between expression of moesin and E-cadherin in BIC-NST(P =0.021)and BDCIS(P =O.032).Conclusion Higher moesin and lower E-cadherin signal transduction is closely related to the recurrence and development of breast carcinoma,therefore moesin and E-cadherin might provide new targets for gene therapy in breast carcinoma.