摘要目的探讨乙型肝炎病毒X蛋白结合蛋白（ HBXIP）在胃癌细胞株AGS中的定位及其过表达与胃癌患者临床病理参数及预后的关系。方法采用免疫荧光技术检测HBXIP在胃癌细胞株AGS的定位，采用免疫组织化学EnVision法检测HBXIP在97例胃癌组织、41例癌旁非瘤组织和13名正常胃黏膜上皮组织中的表达，并分析HBXIP过表达与胃癌患者病理参数及预后评估之间的关系。结果免疫荧光、免疫组织化学结果显示HBXIP主要定位于胃癌细胞的细胞质，免疫组织化学结果显示 HBXIP 蛋白在胃癌组织中表达阳性率和强阳性率分别为68.0％（66／97）和49.5％（48／97），显著高于癌旁非瘤组织（48.8％，20／41；36.6％，15／41）和正常胃黏膜上皮组织（2／13，1／13；P值均＜0.05）；卡方检验结果显示，HBXIP表达水平与胃癌患者分化程度、有无淋巴结转移密切相关（P值分别为0.007和0.041）。 Kaplan-Meier生存分析表明，HBXIP高表达胃癌患者总生存期显著低于HBXIP低表达的患者（ P＝0.015）。结论 HBXIP表达主要定位于细胞质，表达水平与胃癌的发生发展及预后密切相关。 HBXIP表达水平可成为预测胃癌患者预后的重要指标。

abstractsObjective To investigate the localization of HBXIP protein over-expression in gastric adenocarcinoma , and its prognostic significance.Methods HBXIP localization was detected by immunofluorescence in AGS gastric cancer cell line , and by immunohistochemical staining in 97 gastric adenocarcinomas , 41 adjacent non-tumor tissues and 13 gastric adenoma tissues.Correlation between HBXIP expression and clinicopathological features of gastric cancer patients was evaluated by Chi -square and Fisher′s exact tests.Overall survival rates were calculated using Kaplan-Meier method.Results HBXIP was mainly expressed in the cytoplasm of gastric cancer.The positive and strongly positive expression rates of HBXIP protein in gastric cancers were 68.0% ( 66/97 ) and 49.5% ( 48/97 ) respectively , and were significantly higher than those in adjacent non-tumor tissues (48.8%,20/41;36.6%, 15/41) or gastric adenomas(2/13,1/13;all P<0.05).HBXIP expression correlated significantly with tumor differentiation and lymph node status (P=0.007;0.041).Kaplan-Meier survival analysis showed that the overall survival rate was significantly lower in gastric cancer patients with high HBXIP expression ( P =0.015 ) . Conclusions HBXIP expression in gastric cancer is mainly expressed in cytoplasmic , and the expression level is closely related to the prognosis.HBXIP expression status may potentially be used as an important prognostic indicator for gastric cancer .