摘要目的 检测T细胞抗原受体(TCR)标志物βF1及TCRγ在T淋巴母细胞淋巴瘤/白血病(T-LBL/ALL)中的表达情况,并探讨其临床病理特征.方法 收集2010至2016年广东省人民医院病理科51例确诊为 T-LBL/ALL 的病例,采用免疫组织化学 EnVision 法,检测 βF1及 TCRγ 在T-LBL/ALL表达情况并进行统计学分析.结果 51例T-LBL/ALL中儿童及青少年患者13例,成人患者38例.βF1及TCRγ在 T-LBL/ALL的表达率分别为27.5%(14/51)及15.7%(8/51).成人患者(≥18岁)在αβ T-LBL/ALL、TCR 沉默型 T-LBL/ALL 及 γδ T-LBL/ALL 占比分别为7/14、79.3% (23/29)及8/8,其差异具有统计学意义(P=0.023).而性别、乳酸脱氢酶值、骨髓侵犯及临床分期在3组中差异均无统计学意义(P>0.05).8例 γδ T-LBL/ALL 中6例为 CD4-/CD8-表型,14例 αβ T-LBL/ALL中7例为CD4+/CD8+表型.CD4/CD8表达情况在3组中差异有统计学意义(P<0.01).结论 γδ T-LBL/ALL仅发生于成人患者,以CD4-/CD8-表型为主;αβ T-LBL/ALL各年龄阶段均可发生,儿童及青少年患者发生率更高,以CD4+/CD8+表型最多见.

abstractsObjective To evaluate the expression of βF1 and T cell receptor(TCR)γ in T lymphoblastic lymphoma/leukemia(T-LBL/ALL),and investigate the clinicopathological features. Methods Fifty-one cases of T-LBL/ALL were collected at Guangdong General Hospital from 2010 to 2016,the expression of βF1 and TCRγ was assessed by immunohistochemistry. Results There were 13 cases of children and adolescents, and 38 cases of adults. The expression rates of βF1 and TCRγ were 27.5% (14/51)and 15.7%(8/51)respectively. The proportion of adults in αβ T-LBL/ALL,TCR-silent T-LBL/ALL and γδ T-LBL/ALL was 7/14,79.3%(23/29)and 8/8 respectively,and the difference was significant (P=0.023).There was no statistical difference in sex,LDH,bone marrow involvement and Ann arbor stage among these three groups(P>0.05). Γδ T-LBL/ALLs included 6 cases of CD4-/CD8- phenotype,whereas αβ T-LBL/ALL included 7 cases of CD4+/CD8+phenotype. There was significant difference in CD4/CD8 expression among these three groups(P<0.01). Conclusions γδ T-LBL/ALL occurred only in adults, with predominantly CD4-/CD8- phenotype. Αβ T-LBL/ALL occurred more common in children and adolescents,with predominantly CD4+/CD8+phenotype.