摘要目的业已证明，醛固酮可以促进纤维化的形成，血管、脑及肝脏等肾上腺外组织能够合成醛固酮。本实验意在研究醛固酮合成酶基因—CYP11B2在正常与肝纤维化大鼠肝组织中的表达，评价安体舒通的治疗作用。方法取雄性Wistar大鼠160只，体重250克左右，随机分为4组，每组40只。模型组：CCl4油0.25?ml/100?mg皮下注射，每周三次；安体舒通组：CCl4 油注射的同时予以安体舒通20?mg*kg-1*d-1灌胃，1次/日；马洛替酯组：CCl4油注射的同时予以马洛替酯50?mg*kg-1*d-1灌胃，1次/日；对照组：橄榄油皮下注射。于第2、4、6、8和10周宰杀动物，光镜及电镜下动态观察组织学改变，图像分析仪测量胶原面积。RT-PCR和原位杂交检测CYP11B2在正常及纤维化大鼠肝脏的表达。结果原位杂交及RT-PCR显示CYP11B2表达定位于肝星形细胞胞浆，在纤维化形成时表达增强。第四、六周，安体舒通组肝纤维化分级、胶原面积低于模型组（P<0.05），安体舒通组与马洛替酯组无明显差异（P>0.05）。第八、十周，安体舒通组肝纤维化分级,胶原面积高于马洛替酯组（P<0.05）。安体舒通组与模型组相比差异无显著意义（P>0.05）。结论 CYP11B2在纤维化肝脏的星形细胞中表达增强。安体舒通对早期肝纤维化具有一定的治疗作用。

abstractsObjective　To identify aldosterone synthase gene-CYP11B2 mRNA expression in normal and fibrotic liver in rats and evaluate the curative effect of antisterone. Methods　160 Wistar rats weighing about 250?g were divided into 4 groups. In the model group (n=40), the rats were injected with 40% CCl4 (0.25?ml/100?g) subcutaneously three times a week. In the antisterone group (n=40), the rats were injected with 40% CCl4 (0.25?ml/100?g) subcutaneously three times a week. Antisterone equivalent to 20?mg*kg-1*d-1 was given intragastrically (ig). In the malotilate group (n=40), the rats were injected with 40% CCl4 (0.25?ml/100?g) subcutaneously three times a week. Malotilate equivalent to 50?mg*kg-1*d-1 was given ig. In the control group (n=40), the rats were injected with olive oil only. After 2,4,6,8 and 10 weeks, animals were sacrificed, and morphological examination was carried out. The area of collagen was examined with an Image Analyse System. Expression of the aldosterone synthase gene, CYP11B2 mRNA, in fibrotic and normal liver was detected by means of reverse transcriptase polymerase chain reaction (RT-PCR) and in situ hybridization.Results　In situ hybridization and RT-PCR showed that the expression of CYP11B2 mRNA, which localized in the endoplasm of hepatic stellate cells (HSCs), was up regulated when fibrogenesis occurred. Histological observation indicated that the grade of fibrosis and the area of collagen in the antisterone group were less than those in model group before 6 weeks (P<0.05). There was no significant difference between the antisterone and malotilate groups (P>0.05). After that, however, the grade of fibrosis and the area of collagen in the antisterone group were higher than those in the malotilate group (P<0.05). There was no significant difference between the antisterone and model groups (P>0.05).Conclusions　The expression of CYP11B2 mRNA is up regulated in fibrotic liver. Antisterone can have a partial fibrogenesis-inhibiting effect in the early stages.