摘要目的研究幽门螺杆菌vacA基因型、cagA基因,空泡形成细胞毒素和血清CagA抗体与胃十二指肠疾病的关系.方法应用多聚酶链反应方法对62例慢性胃炎、消化性溃疡和胃癌患者分离获得幽门螺杆菌菌株的vacA基因型和cagA基因进行测定;应用Hela细胞方法测定体外空泡形成细胞毒素活性;应用酶免疫测定方法检测同一患者的血清CagA抗体.结果 62株幽门螺杆菌菌株均具有vacA基因,所有菌株的vacA基因型均为sla/m2型.cagA基因的总阳性率为56.45%;慢性胃炎、消化性溃疡和胃癌患者分离获得幽门螺杆菌的cagA基因阳性率分别为55.56%、54.17%和63.64%(P＞0.05).空泡形成细胞毒素的阳性率为37.10%,慢性胃炎、消化性溃疡和胃癌患者分离获得幽门螺杆菌菌株表达空泡形成细胞毒素的阳性率分别为33.33%、29.17%和63.64%(P＞0.05).慢性胃炎、消化性溃疡和胃癌患者血清CagA抗体分别为70.37%、79.17%和40.00%(P＞0.05),血清CagA抗体的总阳性率为68.85%.结论幽门螺杆菌vacA基因型、cagA基因、空泡形成细胞毒素和血清CagA抗体不能预示H.pylori感染个体将更可能发生何种类型胃十二指肠疾病.

abstractsAbstract:Objective To investigate the correlation among vacA genotypes, cagA gene, VacA, serum CagA antibodies of Helicobacter pylori (H. pylori) and gastroduodenal diseases. Methods vacA genotypes and cagA gene of 62 H. pylori strains isolated from patients with chronic gastritis, peptic ulcer and gastric cancer were tested by polymerase chain reaction, and Hela cell assay for VacA activity in vitro. Serum CagA antibodies were measured by EIA method in the same patients.Results All 62 H. pylori strains possessed the vacA gene and vacA genotypes of all strains were type s1a/m2. Total positive rate of cagA gene was 56.45%; the positive rates of cagA gene of H. pylori strains isolated from patients with chronic gastritis, peptic ulcer and gastric cancer were 55.56%, 54.17% and 63.64%, respectively (P>0.05). The total positive rate of VacA was 37.10%; the positive rates of VacA produced by H. pylori strains isolated from patients with chronic gastritis, peptic ulcer and gastric cancer were 33.33%, 29.17% and 63.64%, respectively (P>0.05). The positive rates of CagA antibodies in patients with chronic gastritis, peptic ulcer and gastric cancer were 70.37%, 79.17% and 40.00%, respectively (P>0.05). The total positive rate of CagA antibodies was 68.85%.Conclusion There was no correlation among cagA gene and vacA genotypes of H. pylori, VacA, serum CagA antibodies and various gastroduodenal diseases.