摘要目的我们研究了3个人类结直肠癌细胞系中一组微卫星位点状况和5-氟尿嘧啶(5-FU)敏感性的相关性，该组位点来自或邻近于肿瘤化疗敏感性可能相关的多个因子。方法　LoVo、SW480和SW116细胞系对5-FU的敏感性经MTT方法测定；用免疫组织化学方法检测三细胞系hMSH2、hMLH1表达水平。用PCR-SSLP-银染法分别分析了三个细胞系基因组中10个微卫星位点状况。 结果　比较三个细胞系MTT分析获得的IC50结果发现，对5-FU LoVo细胞系较SW480和SW116更加敏感(分别为：0.8 μmol/L,2.2 μmol/L和1.9 μmol/L,P=0.000)。免疫组织化学检测结果显示hMSH2在SW480和SW1116阳性，百在LoVo阴性；hMLH1在3个细胞系均阳性。PCR-SSLP-银染法结果显示LoVo细胞系在8/10个位点和其余两个细胞系不同，电泳条带有不同程度的增加或位移，而另外的2/10个位点则具有相同的电泳带型。结论　该组微卫星位点和错配修复状况可以一同作为离体人类结直肠癌细胞系对5-FU敏感性的指标，为了深入阐明它们能否作为临床结直肠癌化疗病人药物筛选和预后评价的有效指标，进一步的在体研究和临床资料总结分析很有意义。

abstractsObjective To study the relationship between sensitivity to 5-FU and the status of a panel of microsatellite loci in three human colon cancer cell lines. Methods Cell viability in several concentrations of 5-FU was assessed by the MTT test. Expression of hMSH2 and hMLH1 in LoVo, SW480 and SW1116 cells were analyzed by immunocytochemical staining. Ten mononucleotide and dinucleotide microsatellite loci were analyzed by the PCR-SSLP-silver staining method. Results By MTT assay, it showed that LoVo cells were more sensitive than SW480 and SW1116 cells (0.8 μmol/L, 2.2 μmol/L and 1.9 μmol/L, respectively, P<0.05). By immunocytochemical staining, hMSH2 was expressed in SW480 and SW1116 cells but not in LoVo cells, while hMLH1 was positive in all three cell lines. The PCR-SSLP-silver staining of 10 microsatellite loci revealed that LoVo cells had a different pattern of electrophoretic bands compared with SW480 and SW1116 cells, manifesting both additions and band-shifts. Conclusion Together with hMSH2 and hMLH1, the status of a panel of microsatellite loci may be used as convenient predictors for drug-optimization or prognosis-assessment in colorectal cancer patients before chemotherapy.