摘要目的观察端粒酶基因在乳腺癌前病变-乳腺导管非典型增生及乳腺癌中的表达,探讨其与恶性转化的关系.方法应用原位杂交方法评价端粒酶基因hTR和hTRT在50例乳腺增生组织(其中乳腺单纯性增生6例,轻度非典型增生9例,中度非典型增生12例,重度非典型增生23例)及26例乳腺癌中的表达.结果在乳腺导管单纯性增生组织中端粒酶基因(hTR、hTRTmRNA)呈弱表达(16.6%)或表达阴性.在乳腺导管轻、中度非典型增生组织中端粒酶基因(hTR、hTRTmRNA)呈弱表达(22.2%,11.1%;33.3%,25.0%),在乳腺导管重度非典型增生组织中端粒酶基因(hTR、hTRTmRNA)表达增强(60.9%,52.1%),在乳腺癌组织中端粒酶基因(hTR、hTRTmRNA)呈较强阳性表达(88.5%,80.8%).乳腺导管重度非典型增生组织中的端粒酶基因表达与在轻中度非典型增生、乳腺浸润性导管癌组织中的表达比较差异显著(P<0.05),但与导管内癌表达无显著差异(P>0.05).结论端粒酶基因hTR、hTRT的表达与乳腺导管非典型增生细胞的恶性转化有关,端粒酶的重新激活可能在乳腺癌的组织发生中起作用.

abstractsObjective To investigate telomerase gene expression in precancerous mammary lesion, such as atypical ductal hyperplasia and breast cancer and to study the relationship between expression and malignant transformation.Methods Expression of human telomerase genes (hTR) and human reverse transcriptase gene (hTRT) in 76 cases of mammary tissue was evaluated using in situ hybridization and included 50 cases of mammary hyperplasia, 6 of which were benign hyperplasia, 9 were mild atypical hyperplasia, 12 were moderate atypical hyperplasia, 23 were severe atypical hyperplasia and 26 were mammary cancer. Results The expressions of hTR and hTRT mRNA were much weaker or negative in benign hyperplasia (16.6%, 0), weak to mild-moderate in atypical hyperplasia (22.2%, 11.1%, 33.3%, 25.0%), strong in severe atypical hyperplasia (60.9%, 52.1%), and significantly strong in mammary cancer (88.5%, 80.8%).The difference between mild-moderate atypical hyperplasia, invasive ductal carcinoma and severe atypical hyperplasia was significant (P<0.05) and the difference between severe atypical hyperplasia and intraductal carcinoma was not significant (P>0.05). Conclusion Telomerase genes (hTR and hTRT) expressions are related to the transformation of atypical hyperplasia. Activated telomerase may play a role in mammary cancer development.