摘要目的研究IL-5在人类鼻息肉组织中的含量、分布和表达,探讨其在嗜酸粒细胞聚集微环境控制中的意义和鼻息肉病的概念. 方法采用ELISA法和免疫组化ABC法检测IL-5在40例鼻息肉患者鼻息肉组织中的含量和表达,并以鼻息肉患者和健康鼻下鼻甲粘膜作对照. 结果①鼻息肉组织中IL-5的含量高于鼻息肉患者和健康鼻下鼻甲粘膜者(P<0.05),后两者之间差异无显著性(P>0.05);伴有变应性鼻炎鼻息肉组织中IL-5的含量高于不伴有变应性鼻炎者(P<0.05);鼻息肉组织中IL-5的含量与患者的年龄、性别无关;② IL-5在80.1%的嗜酸粒细胞中表达, 仅有3.7%的淋巴细胞和/或中性粒细胞表达IL-5,其在上皮细胞中则不表达;在所有表达IL-5的细胞中90.9%为嗜酸粒细胞;IL-5在鼻息肉组织的嗜酸粒细胞中的表达强于鼻息肉患者和健康鼻下鼻甲粘膜者(P<0.05),其在后两者中的表达差异无显著性(P>0.05);IL-5在伴有变应性鼻炎鼻息肉组织的嗜酸粒细胞中的表达强于不伴有变应性鼻炎者(P<0.05);IL-5在鼻息肉组织和下鼻甲粘膜的淋巴细胞和中性粒细胞中表达差异均无显著性(P>0.05,P>0.05). 结论 IL-5是鼻息肉组织中嗜酸粒细胞病理学机理中的关键细胞因子.

abstractsObjectives To study the concentration, distribution and expression of IL-5 in nasal polyp tissues and explore its significance in micro-environment differentiation of eosinophil accumulation. Methods The concentration and expression of IL-5 in nasal polyp tissues of 40 patients were determined by ELISA and immunohistochemistry and inferior turbinate mucosa from patients with nasal polyps and healthy volunteers were used as control. Results IL-5 concentration in polyp tissues was significantly higher than that in turbinate mucosa (P<0.05). There was no significant difference in the turbinate mucosae between patients with nasal polyps and healthy volunteers (P>0.05). IL-5 concentrations in polyp tissues were markedly higher in patients with allergic rhinitis compared with those without (P<0.05). IL-5 concentrations had no correlation with age and sex (P>0.05). 80.1% of the eosinophils were positive for IL-5 and 90.9% of IL-5 positive cells were eosinophils. Only 3.7% of lymphocytes and neutrophils were positive for IL-5; IL-5 was not detectable in epithelial cells. IL-5 expression in eosinophils of polyp tissues was remarkably stronger than that of the turbinate mucosa (P<0.05); there was no significant difference in the the turbinate mucosae between patients with nasal polyps and healthy volunteers (P>0.05). IL-5 expression of eosinophils in polyp tissue was significantly stronger in patients with allergic rhinitis compared with those without (P<0.05). There was no significant difference in IL-5 expression in lymphocytes and neutrophils between polyp tissues and turbinate nasal mucosa (both P>0.05). Conclusion IL-5 is the key cytokine in eosinophilic pathologic mechanisms in nasal polyp tissues.