Low Shear Stress Regulating Autophagy Mediated by the p38 Mitogen Activated Protein Kinase and p53 Pathways in Human Umbilical Vein Endothelial Cells

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摘要To the Editor:Autophagy is reported to play a critical role in low shear stress (LSS)-induced endothelial cell injury and the formation of atherosclerotic plaques.[1] However,the corresponding mechanisms remain unclear.[2-8] This study was to investigate the changes and mechanism of LSS-induced autophagy in human umbilical vein endothelial cells (HUVECs).HUVECs were treated with LSS of 5 dyn/cm2 for 0,5,15,30,and 60 min in a parallel plate flow chamber system.Light chain (LC) Ⅱ,LC3 Ⅰ,and p62,p38 mitogen-activated protein kinase (MAPK) and their protein of phosphorylation of p38 (p-p38) were detected with Western blot analysis.The protein levels of p-p53 (ser15) and their distribution were detected by immunofluorescence (IF).

作者单位 Department of Cardiology, Guangzhou Red Cross Hospital, Jinan University affiliated Guangzhou Red Cross Hospital, Guangzhou, Guangdong 510000, China ^[1] Department of Cardiology, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu, China ^[2]

栏目名称 Correspondence

DOI 10.4103/0366-6999.230724

发布时间 2018-06-11

基金项目

This study was supported by grants from the Natural Science Foundation of Guangdong Province the Scientific and Technological Program of Guangzhou City the Natural Science Foundation of Guangdong Province

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