摘要Background:In the current society,infertility related to age has become a social problem.The in vitro fertilization (IVF) success rate in women with poor ovarian response (POR) is very low.Dandelion extract T-1 (DE-T1) is an effective component of the extract from the leaves and stems of Taraxacum officinale,which is one of the medicines used in some patients with POR,but its molecular mechanism remains unclear.Methods:Following IVF,ovarian granulosa cells (GCs) of sixty patients were extracted and divided into normal ovarian response (NOR) and POR groups.GCs were cultured in a dose-dependent and time-dependent manner with DE-T1,proliferation of GCs was determined by Cell Counting Kit-8 assay,and mRNA levels of insulin-like growth factor 1 receptor (IGF-l R),luteotropic hormone receptor (LHR),follicle-stimulating hormone receptor (FSHR),LHR,and CYP19A1 (aromatase) were determined by quantitative polymerase chain reaction.Progesterone and estradiol (E2) concentrations were determined by enzyme-linked immunosorbent assay.Results:The cell viability gradually increased with the progressive increase in the DE-T1 concentration.Compared with the control group (without DE-T1),the mRNA expressions of FSHR,LHR,IGF-1R,and CYP19A1 were upregulated after the addition of DE-T1,especially in the 2.5％ DE-T1 group (P ＜ 0.01).The expression of IGF-1R was upregulated approximately 25 times (24.97 ± 4.02 times) in the POR group with 2.5％ DE-T1.E2 and progesterone levels increased with the increasing DE-T1 concentration.There were highly significant differences in the E2 and progesterone secretion between the NOR and POR groups (P ＜ 0.01).Conclusion:DE-T1 may promote steroid hormone synthesis by promoting GC proliferation and upregulating GC receptor expression,thereby improving ovarian endocrine function.