摘要目的 探讨乙型肝炎疫苗接种后免疫应答与T淋巴细胞亚群活化的关系.方法 采集18例乙型肝炎疫苗接种应答者、22例乙型肝炎疫苗接种无应答者、10例未接受乙型肝炎疫苗接种的健康对照者全血.用流式细胞术对其外周血单个核细胞的表面标志物CD69、CD4、CD8、CD3等进行检测.计算不同T淋巴细胞亚群百分比.并经t检验比较不同T淋巴细胞亚群CD69表达的差异.结果 在应答者中,外周血激活型T辅助淋巴细胞(CD69~+ CD4~+ T淋巴细胞)数明显升高,而CD69~+ CD8~+ T淋巴细胞数则明显减少.应答者、无应答者、对照者外周血CD69~+ CD4~+ T淋巴细胞分别占CD4~+ T淋巴细胞的(1.81±1.33)%、(0.64±0.48)%和(0.56±0.39)%,应答者分别与无应答者和对照者比较.差异均有统计学意义(t=3.444、3.940,均P<0.01).应答者、无应答者、对照者外周血CD69~+ CD8~+ T淋巴细胞分别占CD8~+ T淋巴细胞的(0.43±0.25)%、(3.05±1.69)%和(3.11±2.41)%,应答者分别与无应答者和对照者比较,差异均有统计学意义(t=7.997、5.944,均P<0.01).应答者、无应答者、对照者CD3~+ CD4~+ T淋巴细胞占CD3~+ T淋巴细胞的百分比和CD3~+ CD8~+ T淋巴细胞占CD3~+ T淋巴细胞的百分比比较差异均无统计学意义.结论 乙型肝炎疫苗接种后无应答与Th细胞早期活化不足有关.

abstractsObjective To investigate the relationship between early activation of T lymphocyte subsets and immune responses to hepatitis B surface antigen ( HBsAg) vaccination. Methods Peripheral blood samples were collected from 18 responders to HBsAg vaccination, 22 non-responders and 10 non-immunized healthy controls. Thereafter, the expressions of CD69, CD4, CD8, CD3 on peripheral blood mononuclear cell (PBMC) were determined by flow cytometry. The percentages of different T lymphocyte subsets were calculated. The expressions of CD69 on CD4~+ and CD8~+ lymphocytes were compared among the 3 groups and analyzed by t test. Results CD69~+ CD4~+ T lymphocytes increased and CD69~+ CD8~+ T lymphocytes decreased in the responders to HBsAg vaccination. The percentages of CD69~+ CD4~+ T lymphocytes in CD4~+ T lymphocytes of responders, non-responders and controls were (1.81 ± 1.33)%, (0. 64 ± 0.48)% and (0.56 ± 0. 39)%, respectively. CD69 expression on CD4~+ T lymphocytes in responders was increased as compared to non-responders as well as controls(t = 3. 444 and 3.940. respectively; both P < 0. 01). The percentage of CD69~+ CD8~+ T lymphocytes in CD8~+ T lymphocytes of responders was significantly lower than those of non-responders and controls [(0. 43±0. 25)% vs (3. 05± 1. 69)% vs (3.11± 2.41)%] (t = 7. 997 and 5. 944, respectively; both P<0. 01). The percentages of CD3~+ CD4~+ T lymphocytes and CD3~+ CD8~+ T lymphocytes in responders, non-responders and controls were not statistically different. Conclusion Failure of early activation of helper T cells may contribute to non response to HBsAg vaccination.