摘要目的 了解微小RNA(miRNA)-3620在抗结核药物诱导肝毒性(anti-tuberculosis drug-induced hepatotoxicity, ATDH)患者血浆中的表达水平.方法 ATDH和非ATDH患者各35例,实时荧光定量PCR检测两组患者血浆中miRNA-3620的相对表达量,两组间比较采用t检验;根据miRNA-3620水平绘制受试者工作特征曲线,评估血浆中miRNA-3620在ATDH诊断中的价值.结果 ATDH患者和非ATDH患者血浆中miRNA-3620表达量分别为1.65±1.43和0.71±0.45,差异有统计学意义(t=3.703,P<0.01).miRNA-3620表达水平的最佳截点为1.15,曲线下面积为0.71(95%CI:0.43~1.45),miRNA-3620诊断ATDH的敏感度为60.0%、特异度为82.9%、阳性预测值为77.8%、阴性预测值为67.4%,正确诊断ATDH 21例,非ATDH 29例,准确度为71.4%.结论 ATDH患者血浆中miRNA-3620表达水平明显升高.

abstractsObjective To investigate the expression of microRNA (miRNA)-3620 in the plasma of patients with anti-tuberculosis drug-induced hepatotoxicity (ATDH).Methods A total of 35 patients with ATDH and 35 non-ATDH paired individuals were included in this study.Plasma miRNA-3620 levels were detected using real-time Polymerase chain reaction.Comparison between two groups was done with t test.Receiver operation characteristic (ROC) curve analysis was performed to determine the diagnostic value of miRNA-3620 in ATDH.Results The relative expression of plasma miRNA-3620 of patients with ATDH and non-ATDH were 1.65±1.43 and 0.71±0.45, respectively, with significantly statistical difference (t=3.703, P<0.01).The cut off value of miRNA-3620 expression was 1.15 and the area under ROC curve were 0.71(95% CI: 0.43-1.45).Based on this cutoff value, the sensitivity and specificity of miRNA-3620 in diagnosing ATDH were 60.0% and 82.9%, respectively;the positive predictive value was 77.8% and the negative predictive value was 67.4%.Twenty-one ATDH cases and 29 non-ATDH cases was correctly diagnosed, with the accuracy of 71.4%.Conclusion The expression of miRNA-3620 in plasma is significantly increased in ATDH patients.