摘要目的 探讨静脉输注高氧液对颅脑交通伤家兔神经细胞凋亡的影响. 方法 筛选合适的撞击速度制作家兔颅脑交通伤模型成功后,每日分别给予高氧液25,50,75,100 ml治疗.观察各组动物的线粒体呼吸控制率(RCR)、膜电位(MMP)、神经细胞凋亡率的改变,以及神经损害程度评分(NSS)的变化. 结果 经过筛选,以72 km/h的撞击速度制作的脑损伤模型为研究对象,与未干预组相比,对照组和治疗组脑线粒体MMP、RCR、磷氧比(P/O)及氧化磷酸化率(OPR),神经细胞凋亡率和NSS差异均有统计学意义(P<0.05).100 ml高氧液治疗组的MMP、RCR、P/O及OPR水平要优于75 ml高氧液治疗组(P<0.05),而神经细胞凋亡率和NSS则低于75 ml高氧液治疗组,但差异无统计学意义(P>0.05). 结论 静脉输注高氧液能降低颅脑损伤后神经细胞凋亡率,通过干预线粒体功能可能是高氧液影响神经细胞凋亡从而改善预后的重要途径之一.

abstractsObjective To investigate the effect of hyperoxie liquid on neuron apoptosis in rabbit with traffic brain injury. Methods Rabbit models of brain injury were established by screening suit-able striking intensity and supplied respectively with hyperoxic liquid for 25, 50, 75 and 100 ml each day to observe the respiratory control rate(RCR) of mitochondria, mitochondria membrane potential (MMP), apoptosis rate of neurocytes and NSS grade.Meanwhile, non-intervention group and control groups were set.Results The rabbit model made by striking velocity of 72 km/h was selected as the study object.MMP, RCR, phosphate/oxygen ratio(P/O), oxygenated phosphate rate(OPR) and apopto-sis rate of neurocytes and NSS grade in control and treatment group showed statistical difference compared with non-intervention group(P<0.05).MMP, RCR, P/O and OPR in 100 ml hyperoxic liquid treat-ment group were higher than those in 75 ml hyperoxie liquid treatment group(P<0.05), but the apopto-sis rate of neurocytes and NSS grade showed no statistical difference(P>0.05). Conclusion Intra-venous hyperoxic liquid infusion can decrease apoptosis rate of neurocytes, as indicates that hyperoxic liq-uid affects apoptosis of neurocytes and hence ameliorates prognosis mainly by intervening respiratory func-tion of mitechondria.