摘要目的 通过体外实验对表面进行阶跃式阳极氧化技术改性后的镁合金材料(AZ-1和AZ-3)进行生物相容性评价. 方法用混合酶消化法分离SD大鼠颅盖骨成骨细胞并进行培养;将成骨细胞分别与上述新型材料体外复合培养,应用扫描电镜、四唑盐比色试验(MTT法)及碱性磷酸酶活性测定,对材料上复合堵养的细胞进行形态学和功能测定.利用新鲜兔血对材料进行溶血试验. 结果 成骨细胞在新型医用镁合金材料上可良好地黏附、增殖和生长.细胞的活性和碱性磷酸酶活性未受到材料的影响;溶血率小于5%,有良好的血液相容性. 结论 新型的医用镁合金材料(AZ-1和AZ-3)初步显示了良好的体外生物相容性,有望成为一种新的骨科内植物材料.

abstractsObjective To evaluate the in vitro biocompatibility of new biomaterials (AZ-1 and AZ-3) which result from film oxidation of AZ9ID by step anodization. Methods Osteoblastic cells were harvested by sequential enzyme digestion from calvaria of Sprague Dawhy rats and cultured in vitro onto the magnesium alloys AZ-1 and AZ-3 respectively. The morphology and function of the osteoblastic cells were observed by scanning electron microscopy, MTT assay and alkaline phosphatase activity assay. The bio-materials were also tested by in vitro hemolytic test with fresh cony blood. Results The osteoblastic cells adhered to, and proliferated and grew normally and perfectly on surface of the biomedical magne-sium alloys. Their cellular activity and alkaline phosphatase activity were not influenced by the new biomedical material. The in vitro hemolytic test showed that the hemolytic rates of all samples were lower than 5 %. Conclusion Since the new magnesium alloys seem to possess good biocompatibility in vitro, it may be a new type of material as bone implant.