摘要目的 探讨脂肪肝与正常肝内VX2移植瘤超声造影(CEUS)定量参数与肿瘤血管生成的相关性.方法 建立兔脂肪肝与正常肝内VX2移植瘤模型.应用QontraXt超声造影定量分析软件分析两组肝背景及其内VX2移植瘤.免疫组化及荧光定量PCR技术检测肿瘤微血管密度(MVD)、血管内皮生长因子(VEGF)蛋白及基因表达水平,并与CEUS各定量参数进行相关性分析.结果 ①两组肝内VX2移植瘤MVD、VEGF蛋白表达水平差异均无统计学意义(P＞0.05).两组肝内VX2移植瘤VEGF基因表达水平差异无统计学意义(P＞0.05).脂肪肝背景VEGF基因表达水平较正常肝背景低,差异有统计学意义(P＜0.05).②兔脂肪肝及正常肝内VX2移植瘤MVD均与CEUS峰值强度呈正相关(分别为r=0.494,P＜0.05;r=0.591,P＜0.01).两组肝内VX2移植瘤VEGF蛋白表达水平均与CEUS各定量参数无相关性(P＞0.05).③兔脂肪肝组、正常肝组VX2移植瘤MVD均与VEGF蛋白表达水平呈正相关(分别为r=0.508,P＜0.05;r=0.570,P＜0.01).结论 脂肪肝与正常肝内VX2移植瘤MVD、VEGF蛋白及基因表达无差异.两组肝内VX2移植瘤CEUS峰值强度均与MVD呈正相关,超声造影可以间接提示肝恶性肿瘤MVD表达水平,反映肿瘤血管生成情况.

abstractsObjective To comparative analysis the rabbit VX2 tumors angiogenesis in fatty liver and normal liver,and investigate the correlation of contrast-enhanced ultrasound (CEUS) parameters and their angiogenesis.Methods Rabbit models of fatty liver and normal liver with implanted VX2 tumors were established.Two groups of hepatic backgrounds and VX2 tumors were analyzed by QontraXt quantitative software of CEUS.The expression level of tumor microvascular density (MVD) and vascular endothelial growth factor (VEGF) were detected with immunohistochemical techniques and real-time fluorescence quantitative PCR (FQ-PCR).Results ①No significant difference was found in MVD,VEGF protein and gene expression between VX2 tumors in normal liver and fatty liver.The expression of VEGF gene in fatty liver parenchyma were lower than normal liver parenchyma (P ＜0.05).②There was positive correlation between MVD and peak intensity (r =0.494,P ＜0.05; r =0.591,P ＜0.01) both in fatty liver and normal liver VX2 tumors.The expression of VEGF protein were not correlated with TIC parameters (all P ＞ 0.05).③The MVD had positive correlation with expression of VEGF protein in fatty liver and normal liver VX2 tumors (r =0.508,P ＜0.05; r =0.570,P ＜0.01).Conclusions The expression of MVD and VEGF had no significant difference between fatty liver and normal liver VX2 tumors.The peak intensity of VX2 tumors CEUS had positive correlation with MVD both in fatty liver and normal liver,which can indirectly reflect MVD expression level and help to evaluate tumor angiogenesis.