摘要目的 观察母鼠妊娠及哺乳期补充不同剂量二十二碳六烯酸(DHA)微藻油(DMO)对卵清蛋白(OVA)致敏仔鼠的影响.方法 据Balb/C雌鼠妊娠及哺乳期补充DMO量不同将其仔鼠(66只)分为4组,即低剂量组(0.7％ DMO,L组,16只)、中剂量组(2.1％ DMO,M组,16只)、高剂量组(3.5％ DMO,H组,17只)和对照组(0DMO,17只);各组仔鼠随机选取8只于21日龄OVA暴露前处死,剩余仔鼠于59日龄OVA反复暴露后处死,高效液相色谱法测仔鼠21、59日龄血清多不饱和脂肪酸(PUFA)水平;HE染色观察空肠绒毛形态;甲苯胺蓝染色计数空肠肥大细胞密度;酶联免疫吸附实验(ELISA)测血清OVA-IgE、粪便总IgA、OVA-IgA、脾脏单个核细胞(SMC)培养上清白介素(IL)-4、干扰素(IFN)-γ水平;实时PCR测仔鼠SMC IL-10、转化生长因子(TGF)-β1 mRNA表达.多组数据之间采用单因素方差分析进行比较,组间两两比较采用LSD法.结果 M、H组与对照组相比,21日龄血清DHA[(108 ±29)、(102±34)比(40±19) μg/ml,F=12.052,P=0.000]和二十碳五烯酸(EPA)[(6.7±2.3)、(7.7±2.0)比(3.9±1.1) μg/ml,F=9.573,P=0.000]水平显著增加;59日龄n-3DHA水平H组仍高于对照组[(17.1±2.9)比(5.9±3.3) μg/ml,F=10.339,P＜0.000];SMC IL-4水平H组[(42 ±9) pg/ml]低于对照组[(53±12) pg/ml,F=2.484,P＜0.05].结论 母鼠妊娠及哺乳期补充高剂量DMO可增加仔鼠DHA水平,持续至成年期,对仔鼠耐受OVA致敏能力的影响有限.

abstractsObjective To study the tolerance to ovalbumin (OVA) in suckling mice whose mothers had different doses of docosahexenoic acid (DHA) microalgae oil (DMO) supplementation during pregnancy and lactation.Method According to different doses of DMO fed to mother mice during pregnancy and lactation,66 suckling mice were divided into four groups.Suckling mice whose mothers were fed with 0.7％ DMO were designated as low dose group (group L) (n =16),2.1％ DMO as middle dose group (group M) (n =16),3.5％ DMO as high dose group (group H) (n =17) and no DMO as control group (n =17).Before exposing to OVA,8 suckling mice were killed in each group at 21-day-old.Remaining suckling mice were killed at 59-day-old after repeated OVA exposure.The serum polyunsaturated fatty acid (PUFA) levels of suckling mice were analyzed by high performance liquid chromatography (HPLC) at the age of 21-and 59-day.Histological examinations of jejunum were performed by HE staining and the mast cells in jejunum were observed by toluidine blue staining.OVA-IgE in serum,total IgA and OVA-IgA in the feces and IL-4 and IFN-γin the supernatants of splenic mononuclear cells (SMC) were measured by ELISA.Real time PCR was performed to identify the gene expression of IL-10,TGF-β1 mRNA in SMC.Differences among groups were compared by one-way AVOVA and that between each group were compared by LSD.Result In group M and H,the serum levels of n-3DHA (108 ± 29) pg/ml;(102 ± 34) pg/'ml vs.(40 ± 19) pg/ml (F =12.052,P =0.000) and n-3 eicosapentaenoic acid (6.7 ± 2.3) μg/ml;(7.7 ± 2.0) μg/ml vs.(3.9 ± I.1) μg/ml(F =9.573,P =0.000) were significantly higher than that in control group at the age of 21-day.The serum levels of n-3DHA were higher in group H (17.1 ±2.9) μg/ml than that in control group (5.9 ± 3.3) μg/ml after repeated OVA exposure at the age of 59-day (F =10.339,P ＜ 0.000).Compared with control group (53 ± 12) pg/ml,the levels of IL-4 in SMC in group H (42 ± 9)pg/ml were lower (F =2.484,P ＜ 0.05).Conclusion The serum levels of DHA in baby mice,whose mothers was fed with DMO during pregnancy and lactation,were significantly increased till adulthood.However,the effect on tolerance to OVA was limited.