摘要目的　用逆转录病毒载体将CD80基因导入人卵巢癌细胞系TYK，观察表达CD80基因的TYK(TYK-hCD80)细胞体外诱导细胞毒T淋巴细胞（CTL）的增殖及其杀瘤活性。方法　用逆转录病毒载体将CD80基因导入TYK细胞，流式细胞仪测定其CD80基因的表达。用TYK-hCD80细胞在抗CD3单克隆抗体(单抗)存在下刺激外周血单个核细胞（PBMC），诱导CTL，3H-胸腺嘧啶核苷掺入法测定其增殖活性，四甲基偶氮唑蓝法测定CTL的杀瘤活性。结果　转染后经G418(geneticin, 是一种氨基糖甙类抗生素)筛选，流式细胞仪检测，CD80基因表达率最高为84.9%。TYK-hCD80、TYK在抗CD3单抗存在下刺激PBMC增殖时，3H-胸腺嘧啶核苷掺入量分别为（40 604±842）、（8 000±594） cpm，两者比较，差异有显著性（P<0.05）。TYK-hCD80体外诱导的CTL较TYK诱导者对TYK细胞的杀伤率显著增高(P<0.05)。 结论　表达CD80基因的卵巢癌细胞在抗CD3单抗存在下能诱导产生CTL，增殖快且有较高的杀伤活性，可能为卵巢癌的免疫基因治疗提供实验依据。

abstractsObjective　To investigate the proliferation and cytotoxicity of cytotoxic T lymphocyte （CTL） induced by ovarian cancer cells transfected with a CD80 gene containing retroviral vector. Methods　The ovarian cancer cell line TYK cells were transfected with retro-virus vector PLXSN-hCD80. After geneticin (G418) selection, the CD80 expression of the transfectants was confirmed by flow cytometry. CTL was induced by co-culture of CD80-expressing TYK cells (TYK-hCD80) and peripheral-blood mononuclear cells (PBMC) in the presence of anti-CD3 monoclonal antibody (McAb). Proliferation of PBMC was measured using 3?H-Thymidine incorporation assay. The lysis activity of CTL toward tumor cells was determined using methyl thiazolyl tetrazolium (MTT) assay. Results　After transfection and G418 selection, the CD80 expression was proved by flow cytometry. The highest rate of CD80 expression was 84.9%. The TYK cell line expressing high CD80 was named TYK-hCD80. In the presence of anti-CD3 McAb,TYK-hCD80 significantly enhanced proliferation of PBMC than TYK cells (3H-Thymidine incorporation, （40 604±842） vs （8 000±594） cpm （P<0.05）. The lysis activity of CTL activated by TYK-hCD80 was higher than that of TYK（P<0.05）. Conclusions　Ovarian cancer cells expressing CD80 can induce the production of CTLs which have high lysis activity and high proliferation rate in the presence of anti-CD3 McAb. This study may provide the experimental basis for immunogenic therapy of ovarian cancer.