摘要目的 探讨不同浓度解脲脲原体血清型3(UU3)在小鼠生殖道中的致病性.方法 将156只昆明小鼠按完全随机设计法分为A、B、c组(每组各48只)及对照组(12只)共4组.将3种不同浓度的UU3接种至各实验组小鼠阴道内,各组的浓度分别为:A组1×107 copy/g,B组1×106copy/g,c组1×105 copy/g;对照组仅接种UU液体培养基.于接种后第1、3、7、14、21、35天每次随机处死A、B、c组各8只及对照组2只小鼠,取宫颈管分泌物,荧光定量PCR(FQ-PCR)技术检测各组UU3的浓度及阳性率;取宫颈、子宫内膜及输卵管组织,光镜下观察其形态,并计算致病率.结果(1)A、B、C 3组UU3总阳性率分别为63%(30/48)、50%(24/48)和17%(8/48),3组间比较,差异有统计学意义(P<0.01).接种后1、3、7、14、21、35 d小鼠的UU3阳性率,A组分别为8/8、7/8、6/8、5/8,4/8和0,B组分别为7/8、5/8、5/8、4/8、3/8和0,C组分别为3/8、2/8、2/8、1/8、0和0;对照组各时间点均为0.接种后1 d的UU3阳性率3组间比较,差异有统计学意义(P<0.05);其他各时间点3组间比较,差异均无统计学意义(P>0.05).(2)在UU3检测阳性者中,A、B、C组的UU3浓度在接种后1、3、7、14、21 d分别为1.70×107、3.75×106和1.45×105copy/g,8.26×10+6、2.56×106和1.07×105copy/g,4.04×106、1.37×106和5.43×104copy/g,2.86×106、6.72×105和4.68×103copy/g,2.41×105、1.12×105和0 copy/g.除接种后21 d外,接种后各时间点A、B、C 3组的UU3浓度比较,差异均有统计学意义(P<0.05);A、B组内接种不同时间点的UU3浓度比较,差异均有统计学意义(P<0.05);C组接种不同时间点的UU3浓度比较,差异无统计学意义(P>0.05).(3)接种后7～35 d,A、B、C 3组的总致病率分别为56%(18/32)、44%(14/32)和6%(2/32),3组间比较,差异有统计学意义(P<0.01);接种后7、14、21、35 d小鼠的致病率,A组分别为5/8、5/8、4/8和4/8,B组分别为4/8、4/8、3/8和3/8,C组分别为1/8、0、1/8和0;对照组各时间点均为0.除接种后14 d外,各时间点A、B、C 3组间致病率比较,差异均无统计学意义(P>0.05).结论在小鼠生殖道局部接种不同浓度的UU3,其致病性不同.当UU3浓度≥1×106copy/g时,其致病性明显增强.

abstractsObjective To study the pathogenicity of ureaplasma urealytieum serotype 3 (UU3) with different concentration in the genital tract of the mice. Methods A total of 156 Kunming mice were divided into 4 groups randomly, including group A, B, C (48 mice in every experimental group) and control group.(12 mice in control group). UU3 at concentration of 1×107eopy/g (group A), 1×106copy/g (group B),1×105copy/g (group C) were inoculated into 48 mice in every experimental group intravaginal]y, in the mean time, culture medium of UU was given into 12 mice in control group. They were neeropsied at 1, 3,7, 14, 21, 35 days of postinoeulatien randomly, which included 8 mice of every experimental group and 2 mice of control group every time, and to detect UU3 expression from cervical secretions by FQ-PCR andobserving the pathogenicity rate in tissues of cervix, endometrium, fallopian tube by light microscope and calculate the morbidity rate. Results (1) The total positive rates of UU3 were 63% (30/48) in group A,50% (24/48) in group B, 17% (8/48) in group C, which showed a significant difference(P<0.01).And at 1,3,7,14,21,35 days of postinoculation, the positive rates of group A were 8/8,7/8,6/8,5/8,4/8 and 0,group B were 7/8,5/8,5/8,4/8,3/8 and 0,group C were 3/8,2/8,2/8,1/8,0 and 0;all mice in control group were zero. At all time points, there were statistical difference in the positive rate among three experimental groups only at 1 day (P<0.05 ). (2) In the positive mice, their UU3 quantity concentration at 1,3,7,14,21 days were 1.70×107, 8.26×106, 4.04×106, 2.86×106,and 2.41 x105 copy/g in group A; 3.75×106, 2.56×106 , 1.37×106, 6.72×105, and 1.12 x 105 copy/g in group B, and 1.45×105,1.07×105, 5.43×104, 4.68×103, and 0 copy/g in group C. There were statistical difference among experimental groups at all time points except 21 days (P<0.05). Comparing the concentration among all time points of every group, both group A and B showed a significant difference(P<0.05) ,group C didn't reach it( P>0.05). (3) The total pathogenicity rates of three groups were significant different at 7-35 days, which were 56% (18/32) in group A, 44% (14/32) in group B, 6% (2/32) in group C (P<0.01 ). And at 7,14,21,35 days of postineculation, the pathogenicity rates in group A were 5/8,5/8,4/8 and 4/8, group B were 4/8,4/8,3/8 and 3/8, group C were 1/8,0,1/8 and 0; all mice in control group were zero, which demonstrated significant difference only at 14 days (P<0.05), no other statistical difference were observed (P>0.05) . Conclusions The pathogenicity of UU3 varies with different concentration in genital tract of mice. When UU3 concentration is more than 1×106 copy/g, the susceptibility to infection was intensified significantly.