摘要目的:通过动物实验评价新型经颈静脉肝内门体分流术（TIPS）穿刺套件的可行性与安全性。方法:普通家猪共13只，通过随机数法随机分入实验组（ n=7）和对照组（ n=6），实验组采用新型TIPS穿刺套件，对照组采用RUPS-100，分别进行经颈静脉经肝内门静脉穿刺实验。术后实验组和对照组各随机选取3只即刻处死，其余均饲养1周后处死。观察术中技术成功率、穿刺次数、手术时间、透视时间、生命体征及操作相关并发症情况，观察术前、术后即刻和随访1周后肝肾功能指标改变，观察术后即刻和术后1周的并发症情况。采用独立样本 t检验、配对 t检验、Mann-Whitney U检验、χ 2检验或Fisher确切概率法对观察指标进行比较。 结果:实验组和对照组的技术成功率均为100%。实验组和对照组术中尝试门静脉穿刺次数分别为2.0（1.0，5.0）和2.0（1.0，5.5）次，手术时间分别为（47.7±20.6）和（52.5±28.0）min，透视时间分别为（725.1±489.2）和（763.7±562.4）s，差异均无统计学意义（ P>0.05）。实验组术前、术后即刻及随访1周后的肝肾功能指标与对照组相比差异均无统计学意义（ P均>0.05）。实验组有2例发生非目标穿刺，对照组有2例发生非目标穿刺，差异无统计学意义（ P>0.999）。术后即刻解剖时发现实验组和对照组均有2例发生肝门周围小血肿。2组均未发生腹腔出血等严重并发症。 结论:新型TIPS穿刺套件行实验动物经颈静脉肝内门静脉穿刺具有可行性和安全性。

abstractsObjective:To evaluate the feasibility and safety of a novel transjugular intrahepatic portosystemic shunt (TIPS) puncture set for transjugular intrahepatic portal puncture in swine.Methods:Thirteen domestic swine were randomly divided into experimental group ( n=7) and control group ( n=6) and received transjugular intrahepatic portal puncture with the novel puncture set and the R?sch-Uchida Transjugular Liver Access Set (RUPS-100), respectively. Three swines in each group were randomly selected and sacrificed immediately after the procedure and the rest were sacrificed 1 week later. The intraoperative technical success rate, puncture attempts, procedure time, fluoroscopy time, vital signs and occurrence of complications related to the procedure as well as the changes of liver and kidney function before, immediately after and 1 week after the procedure were compared between two groups. Independent sample t test, paired t test, Mann-Whitney U test, chi-square test or Fisher′s exact test were performed to compare the differences between groups. Results:The technical success rate was 100% for both groups. No significant difference in portal puncture attempts [2.0 (1.0, 5.0) vs. 2.0(1.0, 5.5), P>0.999], procedure time [(47.7±20.6) vs. (52.5±28.0)min, P=0.729] and fluoroscopy time [(725.1±489.2) vs. (763.7±562.4)s, P=0.897] was observed between two groups. In addition, no significant difference of the major liver and kidney function between two groups before, immediately after and 1 week after procedure ( P>0.05 at all time points). No significant difference of the major liver and kidney function change in two groups during perioperative period was identified (all P>0.05). Furthermore, no significant difference of non-target puncture occurrence was observed between two groups ( P>0.999). In the meantime, no significant difference of occurrence of small hematomas around hepatic hilar was discovered among swine sacrificed immediately after procedure between two groups ( P>0.999). No other major complications were identified in either group. Conclusion:The novel TIPS puncture set is feasible and safe to perform transjugular intrahepatic portal puncture in swine.