摘要目的 探讨XRCC1单核苷酸多态性与鼻咽癌放疗敏感性的关联性.方法 放疗前采鼻咽癌患者静脉血,用多聚链酶反应-高温连接酶反应(PCR-LDR)进行XRCC1Arg399GIn基因的分型,观察各型放疗中及放疗后3个月的疗效.结果 放疗中期G1n/G1n基因型的肿瘤退缩率为0.855±0.026,显著高于Are/G1n基因型的0.386±0.183和Arg/Arg基因型的0.377±0.169,差异有统计学意义(F=11.16,P=0.0003);XRCCI Codon399G1n/G1n型放疗后3个月CR达100%,Arg/G1n型为76.2%,Are,/Arg型为66.7%.三者差异无统计学意义.结论 XRCC1Codon399基因型可以预测鼻咽癌放疗中期的局部疗效.

abstractsObjective To find the intrinsic influencing factor of radiosensitivity, and to detect the polymorphisms of XRCC1 of the patients suffering from nasopharygeal carcinoma (NPC) and the different short-term curative effect of radiotherapy. Methods XRCC1 Codon Arg399Gln was determined by polymerase chain reaction and ligase detection reaction(PCR-LDR), and the effect of radiotherapy of NPC on mid-therapy and 3 months after finishing radiation were observed. Results On mid-radiotherapy, the retraction rate of Gln/Gln genotype was 0.855 ± 0.026, which was higher than that of Arg/Gln (0.386 ± 0.183) and Arg/Arg genotypes (0.377±0.169) (F = 11.16, P = 0.0003). The CR rate of 3 months after radiotherapy of the case with Gln/Gln was 100% , Arg/Gln was 76.2% , Arg/Arg was 66.7% ( P > 0.05). Conclusion Polymorphisms of XRCClArg399Gln could be a predictor for the radiotherapy effect of NPC on mid-radiotherapy.