摘要目的 探讨羧酸富勒烯C_3(C_3)在辐射防护和辅助放疗方面的应用.方法 以不同浓度C_3与AHH-1、K562细胞共孵育,用CCK-8法、锥虫蓝试验检测C_3对细胞活力和存活率的影响,用Annexin-V/PI染色、流式细胞分析法研究照射后细胞凋亡和细胞周期的变化.结果 C_3对AHH-1细胞活力几乎无影响(存活率大于95%),但600 mg/L的C,使K562细胞存活率明显降低(82%).100 mg/L C,用药照射组与单纯照射组相比,4 Gy照射后AHH-1细胞的存活率显著升高(71.3%、90.3%),细胞凋亡率明显下降(26.3%、12.6%);而受照射24 Gy的K562细胞存活率却呈现下降趋势(69.4%、66.1%),不同剂量照射后细胞凋亡率反而增加.细胞周期分析结果显示,C,处理组AHH-1细胞12 Gy辐射后G_2期阻滞(27.2%)与单纯照射组(40.8%)相比减轻;而K562受照细胞则反而加重.结论 C_3对AHH-1细胞具有较好的辐射防护作用,而对K562细胞则表现为抑制细胞增殖,促进辐射诱导的细胞凋亡并加重G_2期阻滞.

abstractsObjective To investigate the application prospective of carboxyfullerene C_3 as a radioprotectant or assistant for tumor radiotherapy.Methods Different concentrations of C_3 were incubated with K562 and AHH-1 cell,CCK-8 assay and trypan blue rejection test were performed to examine the influence of C_3 on the cell viability.Annexin V/PI staining and flow cytometry assay were applied to assess the cell cycle and apoptosis after 7-ray irradiation.Results C_3 showed little toxicity to AHH-1 cell with the survival rate over 95% ,but 600 mg/L of C_3 markedly inhibited the growth of K562 cell (82%) .Pretreatment of 100 mg/L C_3 significantly increased the survival rate of AHH-1 cell after 4 Gy irradiation compared with the single radiation group(71.3% vs 90.3%) ,but decreased the apoptosis rate (26.3% vs 12.6%) ,while the survival rate of K562 cell was decreased and the apoptosis rate was elevated with the increase of C_3 concentration.Moreover,the cell cycle analysis revealed the G_2 phase block in AHH-1 cell after radiation exposure was mitigated by C_3 pretreatment,but that in K562 cell was aggravated.Conclusions C_3 has good radioprotective effects on AHH-1 cells.For K562 cell,C_3 could inhibit the cell proliferation,promote the radiation induced apoptosis and aggravate the G_2 phase block.