摘要目的观察白藜芦醇对小鼠产生的辐射致死效应和对放射性小肠损伤的保护作用.方法在辐射致死效应实验中,45只小鼠按随机数字表法分为对照组、单纯照射组和照射给药组,每组15只.对照组小鼠给予假照射,单纯照射组和照射给药组小鼠腹部接受7.2 Gy照射.照射给药组小鼠在照射前24 h灌胃给药1次,以后每日给药1次(共5d);对照组、单纯照射组小鼠按照射给药组方法予同等量载体灌胃.观察小鼠30 d生存率.放射性小肠损伤保护实验中,24只小鼠,按体重随机分为对照组、单纯照射组和照射给药组,每组8只.照射和给药方法与辐射致死效应实验的照射和给药方法相同,小鼠腹部照射剂量为6.5 Gy.照射24 h后处死小鼠并取小肠组织进行HE及免疫组织化学染色.结果照射给药组小鼠30 d生存率比单纯照射组提高33.3％.与单纯照射组相比,照射给药组的小肠隐窝细胞凋亡细胞数量下降(t=17.35,P＜0.05),Ki67表达明显升高(t=13.62,P＜0.05).结论 白藜芦醇可以提高受照小鼠生存率,减少辐射诱导小肠隐窝细胞凋亡,提高小肠隐窝细胞增殖能力,对放射性肠炎具有一定保护作用.

abstractsObjective To study the protective function of resveratrol on radiation-induced small intestine injury and lethal effect in mice.Methods Mice were randomly divided into three groups:irradiation (IR) control,IR only,and IR + resveratrol.15 mice each group were irradiated on abdomen with 7.2 Gy γ-rays for cell lethal assay and 8 mice each group were irradiated with 6.5 Gy for small intestine injury assay.For the IR + resveratrol group,the mouse was given resveratrol by intragastric administration 24 h before irradiation and then was fed with resveratrol daily for 5 days.The control and IR alone groups were fed with placebo.After 30 days of IR,mouse survival rate was detected.For small intestine injury experiments,24 h after IR,the mice were terminated and the small intestines were treated with HE and immunohistochemical staining.Results Compared with the irradiation group,resveratrol increased mouse survival by 33.3％,decreased apoptosis in intestinal crypt cells (t =17.35,P ＜ 0.05),and increased Ki67 expression (t =13.62,P ＜ 0.05).Conclusion Resveratrol could protect small intestine injury from ionizing irradiation.